Intestinal Differentiated Cells - Methods and Protocols
Intestinal Differentiated Cells - Methods and Protocols
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肠道分化细胞 - 方法和方案
DOI：
10.1007/978-1-0716-3076-1_3
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Marva G
中科院分区：
文献类型：
--
作者：
Marva G
电细胞基质阻抗传感技术（ECIS）可用于检测和监测肠道细胞的行为。所提出的方法旨在在短时间内取得成果，并且是针对结肠癌细胞系量身定制的。先前已报道肠癌细胞的分化受视黄酸（RA）调节。在这里，结肠癌细胞在用RA治疗之前在ECIS阵列中培养，并且在治疗后监测对RA的任何响应变化。ECIS记录了阻抗对处理和溶剂的响应变化。这种方法是一种记录结肠细胞行为的新方法，为体外研究开辟了新的途径。
The technique electric cell-substrate impedance sensing (ECIS) can be used to detect and monitor the behavior of intestinal cells. The methodology presented was designed to achieve results within a short time frame, and it was tailored to use a colonic cancer cell line. Differentiation of intestinal cancer cells has previously been reported to be regulated by retinoic acid (RA). Here, colonic cancer cells were cultured in the ECIS array before being treated with RA, and any changes in response to RA were monitored after treatment. The ECIS recorded changes in impedance in response to the treatment and vehicle. This methodology poses as a novel way to record the behavior of colonic cells and opens new avenues for in vitro research.