Design of novel electron transfer mediators based on indophenol derivatives for lactate sensor
Design of novel electron transfer mediators based on indophenol derivatives for lactate sensor
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DOI：
10.1016/s0956-5663(01)00288-3
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发表时间：
2002-04-01
影响因子：
12.6
通讯作者：
Ohwa, M
中科院分区：
文献类型：
--
作者：
Hirano, K;Yamato, H;Ohwa, M
在水溶液中溶解度有限的新型吲哚酚衍生物作为乳酸氧化酶和乳酸传感器的优良电子转移介质，具有较高的灵敏度和良好的耐久性。为了防止介质从传感器中浸出，这些低水溶性介质由吲哚酚的o -烷基化或酰化制备。因此，发现吲哚酚衍生物的特征性取代基效应如下：(1)酚羟基的修饰提高了传感器的耐久性；(2)苯酚环2,6位氯基团的存在提高了传感器的灵敏度；(3)在喹啉基团上引入取代基导致耐久性恶化。使用这些衍生物制成的基于介质的乳酸传感器几乎不受氧浓度的影响，显示出低水平的干扰效应，并且具有相当长的耐用性。(C) 2002 Elsevier Science B.V.版权所有
Novel indophenol derivatives which have limited solubility in water solution functioned as excellent electron-transfer mediators for lactate oxidase and a lactate sensor, showing good sensor performance, including a high sensitivity and good durability. These less water-soluble mediators were prepared by the O-alkylation or acylation of indophenols in order to prevent the mediator being leached from the sensor. Consequently the characteristic substituent effects of indophenol derivatives were found to be follows, (1) modification of the phenolic OH group improved sensor durability; (2) the presence of chloride groups at the 2,6-positions of the phenol ring improved the sensitivity of the sensor; (3) the introduction of substituents at the quinoide moiety led to a deterioration in durability. A mediator based lactate sensor using these derivatives was found to be nearly independent of oxygen concentration, showed a low level of interference effect, and a quite long durability. (C) 2002 Elsevier Science B.V. All rights reserved.