Integration of transcriptomic and proteomic approaches unveils the molecular mechanism of membrane disintegration in Escherichia coli O157:H7 with ultrasonic treatment
Integration of transcriptomic and proteomic approaches unveils the molecular mechanism of membrane disintegration in Escherichia coli O157:H7 with ultrasonic treatment
复制标题
DOI：
10.1016/j.scitotenv.2021.148366
复制
发表时间：
2021-06-15
影响因子：
9.8
通讯作者：
Guo, Mingming
中科院分区：
文献类型：
--
作者：
He, Qiao;Liu, Yanhong;Guo, Mingming
超声波杀菌在污水处理中的研究已在表型水平上进行了多年，但其分子失活机理尚不清楚。在这里，大肠杆菌O 157：H7超声波处理的反应进行了研究，使用RNA测序（RNA-Seq）和串联质量标签（TMT）为基础的定量蛋白质组学方法。结果显示，770个基因和201个蛋白质在超声处理后发生了显著变化。此外，整合转录组学和蛋白质组学分析揭示了一组59个基因或蛋白质在超声处理的细胞中差异表达，提供了对超声场的细胞反应的概述。生物信息学分析表明，与脂质不对称性保持和外膜稳态维持有关的基因和蛋白（包括磷脂代谢、脂多糖合成和转运以及脂肪酸代谢）表达上调。因此，我们提出细胞膜脂质（包括脂多糖、磷脂和脂肪酸）代谢紊乱是细菌在超声波胁迫下面临的主要挑战之一。本研究首次从多组学角度提出了超声波诱导细胞膜崩解的新机制，为进一步阐明超声波场的分子失活机制迈出了重要一步，并为超声波技术在水体病原菌控制中的应用提供了理论基础. (C)2021爱思唯尔有限公司版权所有。
Ultrasonic disinfection in wastewater treatment has been studied for years at the phenotypic level, while the un-derstanding of the molecular inactivation mechanism is still not clear. Here, the responses of Escherichia coli O157:H7 to ultrasound treatment were investigated using RNA sequencing (RNA-Seq) and tandem mass tags (TMT) based quantitative proteomics methods. The analyses revealed that 770 genes and 201 proteins were sig-nificantly changed upon ultrasound treatment. Moreover, the integrated transcriptomic and proteomic analyses uncovered a set of 59 genes or proteins were differentially expressed in ultrasound-treated cells, providing an overview of the cellular responses to ultrasonic field. According to the bioinformatic analyses, genes and proteins that may be involved in lipid asymmetry preservation and outer membrane homeostasis maintenance (including phospholipid metabolism, lipopolysaccharide biosynthesis and transport, and fatty acid metabolism) were spe-cifically up-regulated. Therefore, we proposed that the metabolism disorder of cellular membrane lipids (lipo-polysaccharide, phospholipid, and fatty acid included) was one of the main challenges for the bacteria upon ultrasonic stress. In this study, we initially proposed a novel mechanism regarding the ultrasound-induced mem-brane disintegration from a multi-omics perspective, which may present an important step toward deciphering the molecular inactivation mechanism of ultrasonic field and provide a theoretical foundation for the application of ultrasound technology for the control of waterborne pathogens. (C) 2021 Elsevier B.V. All rights reserved.