Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
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通过将 Yr30 与染色体臂 4BL 上广泛分散、一致的 QTL 相结合，获得增强的条锈病抗性
DOI：
10.1007/s00122-021-03970-4
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发表时间：
2021-10-19
影响因子：
5.4
通讯作者：
Wu, Jianhui
中科院分区：
文献类型：
--
作者：
Liu, Shengjie;Wang, Xiaoting;Wu, Jianhui
YrFDC 12和PbcFDC共分离于4 BL染色体上，与Yr 30/Pbc 1互作显著提高了小麦的抗条锈性，促进了小麦伪黑壳的形成。持久抗性品种是防治小麦条锈病最常用的手段。通过叠加多个单独作用相对较小的成株抗性（APR）基因可以获得持久抗性。中国小麦品种瑞华520（RH 520）和丰德村12（FDC 12）在不同环境下对条锈病具有部分的APR。以170个重组自交系RH 520 × FDC 12为材料，研究了小麦抗条锈性的遗传基础，并鉴定了与抗条锈性相关的基因组区域。利用55 K SNP芯片进行基因分型，并利用包含性复合区间作图法在2AL、2DS、3BS、4 BL、5 BL（2）、7 BL（2）染色体臂上检测到8个数量性状位点（QTL）。只有RH 520的QYr.nwafu-3BS和FDC 12的QYr.nwafu-4BL.2（为方便起见命名为YrFDC 12）在四个测试环境中一致。QYr.nwafu-3BS可能是多效抗性基因Sr 2/Yr 30。YrFDC 12被定位在对应于12 Mb的2.1-cM间隔中，并且侧翼为SNP标记AX-111121224和AX-89518393。同时含有Yr 30和YrFDC 12的品系表现出比亲本更高的抗性，并表现出分别与Yr 30和YrFDC 12共分离的由基因座Pbc 1和PbcFDC 12控制的伪黑壳（PBC）。基于标记和基于系谱的亲缘关系分析均显示YrFDC 12遗传自创始亲本周8425 B。其他54个小麦品种共享YrFDC 12单倍型。这些结果为在育种中获得高效持久的抗条锈性提供了一种有效的抗条锈策略。
YrFDC12 and PbcFDC, co-segregated in chromosome 4BL, and significantly interacted with Yr30/Pbc1 to enhance stripe rust resistance and to promote pseudo-black chaff development. Cultivars with durable resistance are the most popular means to control wheat stripe rust. Durable resistance can be achieved by stacking multiple adult plant resistance (APR) genes that individually have relatively small effect. Chinese wheat cultivars Ruihua 520 (RH520) and Fengdecun 12 (FDC12) confer partial APR to stripe rust across environments. One hundred and seventy recombinant inbred lines from the cross RH520 × FDC12 were used to determine the genetic basis of resistance and identify genomic regions associated with stripe rust resistance. Genotyping was carried out using 55 K SNP array, and eight quantitative trait loci (QTL) were detected on chromosome arms 2AL, 2DS, 3BS, 4BL, 5BL (2), and 7BL (2) by inclusive composite interval mapping. Only QYr.nwafu-3BS from RH520 and QYr.nwafu-4BL.2 (named YrFDC12 for convenience) from FDC12 were consistent across the four testing environments. QYr.nwafu-3BS is likely the pleiotropic resistance gene Sr2/Yr30. YrFDC12 was mapped in a 2.1-cM interval corresponding to 12 Mb and flanked by SNP markers AX-111121224 and AX-89518393. Lines harboring both Yr30 and YrFDC12 displayed higher resistance than the parents and expressed pseudo-black chaff (PBC) controlled by loci Pbc1 and PbcFDC12, which co-segregated with Yr30 and YrFDC12, respectively. Both marker-based and pedigree-based kinship analyses revealed that YrFDC12 was inherited from founder parent Zhou 8425B. Fifty-four other wheat cultivars shared the YrFDC12 haplotype. These results suggest an effective pyramiding strategy to acquire highly effective, durable stripe rust resistance in breeding.