Methylation increases sodium transport into A6 apical membrane vesicles: possible mode of aldosterone action.
Methylation increases sodium transport into A6 apical membrane vesicles: possible mode of aldosterone action.
复制标题
甲基化增加钠转运至 A6 顶膜囊泡：醛固酮作用的可能模式。
DOI：
10.1126/science.6463652
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发表时间：
1984
期刊：
影响因子：
56.9
通讯作者：
J. Johnson
中科院分区：
文献类型：
--
作者：
S. Sariban;M. Burg;W. Wiesmann;P. Chiang;J. Johnson
当从培养的A6上皮细胞制备的分离的顶膜囊泡在体外与甲基供体S-腺苷甲硫氨酸孵育时，阿米洛利可耐受钠转运的控制率加倍。甲基化抑制剂3-deazaadenosine和S-腺苷高半胱氨酸使S-腺苷甲硫氨酸刺激的钠转运恢复到对照水平。无论是这些代理人，也不影响腺苷钠转运到控制囊泡。在与S-腺苷-[3 H-甲基]甲硫氨酸孵育的囊泡中，膜磷脂和蛋白质都被标记，并且这种标记被deazaadenosine抑制。在用醛固酮处理的A6细胞制备的囊泡中，钠转运是对照值的两倍，S-腺苷甲硫氨酸没有引起任何进一步的转运刺激。在这些囊泡中，脂质和蛋白质甲基化均增加。这些结果表明，甲基化，这增加了阿米洛利敏感的钠转运率参与了醛固酮在上皮细胞顶膜水平的作用。
When isolated apical membrane vesicles prepared from cultured A6 epithelia were incubated in vitro with the methyl donor S-adenosylmethionine, the control rate of amiloride-inhibitable sodium transport was doubled. The methylation inhibitors 3-deazaadenosine and S-adenosyl homocysteine returned the S-adenosyl-methionine-stimulated sodium transport to control levels. Neither these agents nor adenosine affected sodium transport into control vesicles. In vesicles incubated with S-adenosyl-[3H-methyl]methionine, both membrane phospholipids and proteins were labeled, and this labeling was inhibited by deazaadenosine. In vesicles prepared from A6 cells treated with aldosterone, sodium transport was twice the control value and S-adenosylmethionine did not cause any further stimulation of transport. In those vesicles, both lipid and protein methylation were increased. These results suggest that methylation, which increases the rate of amiloride-sensitive sodium transport is involved in the action of aldosterone at the apical membrane level in epithelia.