MOLECULAR ANALYSIS OF REF(2)P, A DROSOPHILA GENE IMPLICATED IN SIGMA RHABDOVIRUS MULTIPLICATION AND NECESSARY FOR MALE-FERTILITY
MOLECULAR ANALYSIS OF REF(2)P, A DROSOPHILA GENE IMPLICATED IN SIGMA RHABDOVIRUS MULTIPLICATION AND NECESSARY FOR MALE-FERTILITY
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DOI：
10.1002/j.1460-2075.1989.tb08508.x
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发表时间：
1989-11-01
期刊：
影响因子：
11.4
通讯作者：
TENINGES, D
中科院分区：
文献类型：
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作者：
DEZELEE, S;BRAS, F;TENINGES, D
黑腹果蝇的 ref(2)P 基因与 Sigma 弹状病毒增殖有关。克隆并测序了许可等位基因。结构基因（3.1 kbp）分为三个外显子。 mRNA 的大小不同。它们仅在第一个外显子的 5'' 末端有所不同。短 mRNA 的上游序列包含经典的启动子元件。长 mRNA 起始位点上游没有适当定位 TATA 和 CAAT 盒，但存在多个重复序列、回文序列和反向 CAAT 盒。这些观察结果，连同短转录本和长转录本的组织依赖性分布，支持了存在至少两类真正起始位点的假设。长的非翻译前导 RNA 区域表明在翻译水平上对基因表达进行控制。预计所有 mRNA 均具有 599 个氨基酸 (76.3 kd) 的相同翻译产物，但体外翻译产物在 SDS-PAGE 中以更高的表观摩尔重量 (115-125 kd) 迁移。推定的 ref(2)P 蛋白包含内部重复序列、可能是蛋白质降解信号的 PEST 区域以及有趣的结构基序，例如锌指和两亲性螺旋。这些后来的基序可能是线粒体前序列。在功能丧失等位基因纯合的不育雄性果蝇的精子细胞中观察到线粒体变性。 ref(2)P 产品的氨基酸序列与数据库中任何已知的蛋白质没有同源性。
The ref(2)P gene of Drosophila melanogaster is implicated in sigma rhabdovirus multiplication. A permissive allele was cloned and sequenced. The structural gene (3.1 kbp) is divided into three exons. The mRNAs are heterogeneous in size. They differ only in the 5'' end of the first exon. The sequence upstream of the short mRNAs contains classical promoter elements. No TATA and CAAT boxes are appropriately positioned upstream of the initiation sites of the long mRNAs, but several repeats, palindromic sequences and inverted CAAT boxes are present. These observations, together with the tissue-dependent distribution of short and long transcripts, support the hypothesis of the existence of at least two classes of genuine initiation sites. The long size of the untranslated leader RNA region suggests a control of gene expression at the translation level. The same translation product of 599 amino acids (76.3 kd) is predicted for all mRNAs, but the in vitro translation product migrates in SDS-PAGE with a higher apparent mol wt (115-125 kd). The putative ref(2)P protein contains internal repeats, PEST regions which may be signals for protein degradation, and interesting structural motifs such as zinc finger and amphiphilic helices. These later motifs could be mitochondrial pre-sequences. The degeneration of mitochondria is observed in the spermatids of sterile male flies homozygous for the loss-of-function alleles. The amino acid sequence of the ref(2)P product shows no homology with any known protein from the data banks.