Lack of gas bladder inflation by the larvae of zebrafish in the absence of an air-water interface
Lack of gas bladder inflation by the larvae of zebrafish in the absence of an air-water interface
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DOI：
10.1111/j.1095-8649.1999.tb00740.x
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发表时间：
1999-11-01
影响因子：
2
通讯作者：
Okutake, K
中科院分区：
文献类型：
--
作者：
Goolish, EM;Okutake, K
与对照组95.1+/-1.9%的气囊充气率相比，封闭腔中保存72 h的斑马鱼幼体的充气率仅为19.1+/-7.7%。在密闭舱中存活10d的幼虫(32%)显著低于在开放舱中存活的幼虫(76%)，而在密闭舱中饲养的幼虫脊柱弯曲程度显著高于在密闭舱中的幼虫。未充气的仔鱼体长变化不大，最终干重约为对照仔鱼的14%。在封闭的气室中发现少量带有充气气囊的幼虫，可能是由于无意中将小气泡引入了两个重复。这些结果表明，接触水-水界面对斑马鱼幼体的正常发育至关重要。而且小的水下球形气体体积可以在有限的程度上用于初始气囊充气。(C)1999年不列颠群岛渔业协会。
Compared to a control gas bladder inflation rate of 95.1 +/- 1.9%, zebrafish Danio rerio larvae 72 h post-fertilization maintained in closed chambers had an inflation rate of just 19.1 +/- 7.7%. Larval survivorship through 10 days in closed chambers (32%) was significantly less than that in open chambers (76%), and the extent of spinal curvature was significantly higher among larvae maintained in closed chambers. Larvae which failed to inflate their gas bladder showed very little change in body length, and had a final dry weight c. 14% of that for control larvae. The small number of larvae with inflated gas bladders found in closed chambers might be attributed to the inadvertent introduction of small bubbles into two replicates. These results indicate that access to an ah-water interface is critical for the normal development of zebrafish larvae. but also that a small, submerged, spherical gas volume may to a limited extent be used for initial gas bladder inflation. (C) 1999 The Fisheries Society of the British Isles.