Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation.
Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation.
复制标题
鉴定负责流产布鲁氏菌 MucR 高阶寡聚体形成的区域并检查其在基因调控中的作用。
DOI：
10.1038/s41598-018-35432-1
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发表时间：
2018
影响因子：
4.6
通讯作者：
Baglivo,Ilaria
中科院分区：
文献类型：
--
作者：
Pirone,Luciano;Pitzer,JoshuaEdison;D'Abrosca,Gianluca;Fattorusso,Roberto;Malgieri,Gaetano;Pedone,EmiliaMaria;Pedone,PaoloVincenzo;Roop2nd,RoyMartin;Baglivo,Ilaria
MucR 是在 α-变形菌中发现的原核锌指蛋白 Ros/MucR 家族的成员，它调节这些细菌与真核宿主成功致病和共生相互作用所需的基因表达。其独特的锌指结构域的结构和功能已得到充分研究，但直到最近才研究了全长蛋白质的四级结构，证明了它们形成高阶寡聚物的能力。本研究的目的是通过光散射分析该蛋白质的缺失和点突变体来鉴定参与高阶寡聚体形成的MucR区域，并确定MucR寡聚化在该蛋白质的调节功能中所起的作用。在这里，我们证明 MucR N 末端的保守疏水区域负责高阶寡聚体的形成，并且 MucR 寡聚化对其在布鲁氏菌中的调节功能至关重要。 MucR 的所有这些特征都是组蛋白样核结构蛋白 (H-NS) 所共有的，因此我们提出 MucR/Ros 家族中的原核锌指蛋白采用类似于 H-NS 蛋白所使用的机制来控制基因表达，而不是充当经典的转录调节因子。
MucR is a member of the Ros/MucR family of prokaryotic zinc-finger proteins found in the α-proteobacteria which regulate the expression of genes required for the successful pathogenic and symbiotic interactions of these bacteria with the eukaryotic hosts. The structure and function of their distinctive zinc-finger domain has been well-studied, but only recently the quaternary structure of the full length proteins was investigated demonstrating their ability to form higher-order oligomers. The aim of this study was to identify the region of MucR involved in higher-order oligomer formation by analysing deletion and point mutants of this protein by Light Scattering, and to determine the role that MucR oligomerization plays in the regulatory function of this protein. Here we demonstrate that a conserved hydrophobic region at the N-terminus of MucR is responsible for higher-order oligomer formation and that MucR oligomerization is essential for its regulatory function inBrucella. All these features of MucR are shared by the histone-like nucleoid structuring protein, (H-NS), leading us to propose that the prokaryotic zinc-finger proteins in the MucR/Ros family control gene expression employing a mechanism similar to that used by the H-NS proteins, rather than working as classical transcriptional regulators.