Intravitreous VEGF-A in eyes with massive vitreous hemorrhage
Intravitreous VEGF-A in eyes with massive vitreous hemorrhage
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DOI：
10.1007/s00417-011-1795-5
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发表时间：
2011-12-01
影响因子：
2.7
通讯作者：
Sakamoto, Taiji
中科院分区：
文献类型：
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作者：
Shirasawa, Makoto;Arimura, Noboru;Sakamoto, Taiji
虽然血管内皮生长因子（VEGF）在血清中是丰富的，大量玻璃体积血（VH）的眼内VEGF的浓度是不众所周知的。本研究通过玻璃体切除术获取玻璃体标本，其中12例为无糖尿病视网膜病变（DR）的视网膜前膜，9例为无DR的大面积VH，如年龄相关性黄斑变性、孔源性VH、Terson综合征和巨大动脉瘤破裂。12个样品从增殖DR。VEGF测定与酶联免疫吸附试验（ELISA）。还检查了与肝素一起或不与肝素一起孵育的样品中结合至玻璃体的VEGF的释放。免疫组化法检测VEGF和II型胶原在玻璃体中的分布，增殖期视网膜病变眼VEGF的表达明显高于增殖期视网膜病变眼（821 +/- 949 pg/ml），而非DR伴大面积VH（2.75 +/- 7.5 pg/ml，P < 0.01，卡方检验）或无VH的非DR（小于可检测水平，P < 0.01，卡方检验）在具有大面积VH的眼睛和没有VH的非糖尿病眼睛之间没有统计学显著差异。肝素治疗对玻璃体VEGF浓度无显著影响。免疫组化分析结果显示VEGF主要位于血凝块中，即使有大量VH，可扩散的VEGF在液相中也不会显著增加，主要存在于血凝块中。从VEGF相关病理学的角度来看，单独的VH不应作为玻璃体切除术的适应症。
Although vascular endothelial growth factor (VEGF) is abundant in serum, the intraocular concentration of VEGF in eyes with massive vitreous hemorrhage (VH) is not well-known. The present study was conducted to elucidate the effects of a massive VH on intravitreous VEGF concentration.Vitreous samples were obtained during vitrectomy: 12 samples from eyes with epiretinal membrane without diabetic retinopathy (DR), and nine samples from massive VH with no DR, such as age-related macular degeneration, rhegmatogenous VH, Terson's syndrome and macro-aneurysm rupture. Twelve samples were obtained from proliferative DR. VEGF was measured with an enzyme-linked immunosorbent assay (ELISA). Samples incubated with or without heparin were also examined for the release of VEGF binding to the vitreous body. The localization of VEGF and type II collagen in the vitreous was evaluated from immunohistochemistry.The concentration of VEGF was significantly higher in eyes with proliferative DR (821 +/- 949 pg/ml) than in non-DR with massive VH (2.75 +/- 7.5 pg/ml, P < 0.01, chi-square test) or non-DR with no VH (less than detectable level, P < 0.01, chi-square test) There was no statistically significant difference between eyes with massive VH and non-diabetic eyes without VH. Treatment with heparin did not significantly affect the concentration of vitreous VEGF. VEGF was localized mainly in the clot from the results of an immunohistochemical analysis.Even with a massive VH, diffusible VEGF does not increase significantly in the liquid phase and is principally present in a clot. VH alone should not be an indication for vitrectomy from the point of view of VEGF-related pathology.