Induction of Pluripotency in Adult Unipotent Germline Stem Cells
Induction of Pluripotency in Adult Unipotent Germline Stem Cells
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DOI：
10.1016/j.stem.2009.05.025
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发表时间：
2009-07-02
期刊：
影响因子：
23.9
通讯作者：
Schoeler, Hans R.
中科院分区：
文献类型：
--
作者：
Ko, Kinarm;Tapia, Natalia;Schoeler, Hans R.
具有与胚胎干细胞相似特征的小鼠和人类干细胞已经从睾丸细胞中获得。虽然从小鼠新生睾丸的确定种系干细胞（GSCs）中获得了多能干细胞，但迄今为止仅从小鼠成年睾丸的确定细胞中获得了多能干细胞。在这项研究中，我们描述了从成人单能性GSCs中获得生殖系衍生多能干细胞（gPS）的稳健且可重复的方案。通过体外和体内分化，包括生殖细胞贡献和传播，证实了gPS细胞的多能性。克隆分析表明，gPS细胞确实起源于单能性GSCs。我们提出，转化过程需要GSC培养微环境，这取决于镀GSC的初始数量和培养时间的长短。
Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germ line-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.