DECREASED BACTERIAL ADHERENCE AND BIOFILM FORMATION ON CHLORHEXIDINE AND SILVER SULFADIAZINE-IMPREGNATED CENTRAL VENOUS CATHETERS IMPLANTED IN SWINE
DECREASED BACTERIAL ADHERENCE AND BIOFILM FORMATION ON CHLORHEXIDINE AND SILVER SULFADIAZINE-IMPREGNATED CENTRAL VENOUS CATHETERS IMPLANTED IN SWINE
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DOI：
10.1097/00003246-199505000-00018
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发表时间：
1995-05-01
影响因子：
8.8
通讯作者：
MODAK, S
中科院分区：
文献类型：
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作者：
GREENFELD, JI;SAMPATH, L;MODAK, S
目的：确定磺胺嘧啶银和氯己定浸渍的无菌中心静脉导管（防腐剂）减少细菌粘附和生物膜形成而不产生局部或全身毒性。设计：前瞻性、随机、对照试验。设置：大学教学医院的实验室。受试者：10头远系繁殖的新罕布什尔州猪。干预措施：将未浸渍（对照）和防腐剂浸渍的导管血管内插入猪体内7天。拔管后，评估导管的细菌粘附和生物膜形成，并评估周围组织的毒性体征。检索前，抗菌剂的全身浓度determined.Measurements和主要结果：顺序辊板和ESTA被用来检测中度和紧密粘附的细菌的导管的外表面和腔表面。扫描电镜检测生物膜的存在。对导管周围组织进行组织病理学检查;通过原子吸收和高效液相色谱法测定氯己定、磺胺嘧啶和银的全身浓度。与对照组相比，抗菌导管的外表面和管腔表面上的中度和紧密粘附细菌显著减少（p <0.01），仅检查外表面时，粘附细菌也减少（p <0.01）。扫描电子显微镜检查显示对照导管上有细菌生物膜和粘附，但抗菌导管上没有。有没有异常的组织病理学变化与测试导管，和血清浓度的抗菌剂被证明是在无毒范围内。结论：防腐浸渍导管防止细菌粘附和生物膜形成，并没有产生局部或全身毒性。
Objective: To determine if antiseptic central venous catheters impregnated with silver sulfadiazine and chlorhexidine (antiseptic) reduce bacterial adherence and biofilm formation without producing local or systemic toxicity.Design: Prospective, randomized, controlled trial.Setting: Experimental laboratory in a university teaching hospital.Subjects: Ten outbred New Hampshire pigs.Interventions: Nonimpregnated (control) and antiseptic-impregnated catheters were inserted intravascularly into swine for 7 days. After explantation, the catheters were assessed for bacterial adherence and biofilm formation, and the surrounding tissue was assessed for signs of toxicity. Before retrieval, systemic concentrations of antimicrobials were determined.Measurements and Main Results: Sequential roll plate and centrifuging were used to detect moderately and tightly adherent bacteria on the outer and luminal surfaces of the catheter. The presence of biofilm was detected by scanning electron microscopy. Tissues surrounding the catheters were examined histopathologically; systemic concentrations of chlorhexidine, sulfadiazine, and silver were determined by atomic absorption and high-performance Liquid chromatography. As compared with the controls, antiseptic catheters had significantly (p < .01) fewer moderately and tightly adherent bacteria on outer and luminal surfaces, and fewer adherent bacteria when outer surfaces alone were examined (p < .01). Scanning electron microscopy showed bacterial biofilm and adherence on the control catheters but not on the antiseptic catheters. There were no abnormal histopathologic changes associated with the test catheter, and serum concentrations of the antibacterial agents were shown to be within non-toxic ranges.Conclusion: The antiseptic-impregnated catheters prevented bacterial adherence and biofilm formation and produced no local or systemic toxicity.