Arp3 is required during preimplantation development of the mouse embryo
Arp3 is required during preimplantation development of the mouse embryo
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DOI：
10.1016/j.febslet.2007.11.031
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发表时间：
2007-12-11
期刊：
影响因子：
3.5
通讯作者：
Arnold, Hans-Henning
中科院分区：
文献类型：
--
作者：
Vauti, Franz;Prochnow, Blair Raymond;Arnold, Hans-Henning
利用Arp3基因中的基因陷阱突变研究Arp3在小鼠发育中的作用。杂合子Arp3（WT/GT）小鼠是正常的，然而，纯合子Arp3（GT/GT）胚胎在囊胚期死亡。早期胚胎阶段似乎不受突变的影响，可能是由于母体Arp3蛋白。在E3.5分离的突变胚泡不能在体外继续发育，培养物中缺乏滋养层样细胞的生长，并且滋养层标志物Cdx 2表达水平降低，而内细胞团的标志物继续存在。隐性胚胎致死表型表明Arp3在小鼠早期发育中起着至关重要的作用，可能是在滋养层细胞对着床至关重要时。(c)2007年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant blastocysts isolated at E3.5 fail to continue development in vitro, lack outgrowth of trophoblast-like cells in culture and express reduced levels of the trophoblast marker Cdx2, while markers for inner cell mass continue to be present. The recessive embryonic lethal phenotype indicates that Arp3 plays a vital role for early mouse development, possibly when trophoblast cells become critical for implantation. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.