Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C-elegans embryos
Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C-elegans embryos
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DOI：
10.1242/jcs.022897
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发表时间：
2008-02-01
影响因子：
4
通讯作者：
von Dassow, George
中科院分区：
文献类型：
--
作者：
Baruni, Jalal K.;Munro, Edwin M.;von Dassow, George
棘皮动物受精卵的经典实验研究得出结论，两个星形微管阵列的并列定位的刺激细胞动力学开沟。然而，最近的实验和遗传学研究在秀丽隐杆线虫，果蝇和哺乳动物培养细胞牵连微管的中央纺锤体，与此相关的调节蛋白，这表明沟诱导的必要条件可能不同，从一个动物细胞到另一个。我们使用显微操作和激光显微外科技术，以三种方式，在C。线虫胚胎细胞在环形细胞中，我们观察到，沟槽启动星形微管阵列并置，并在皮层最接近中央纺锤体。我们发现，双核细胞成功地不仅横跨纺锤体，但也不相连的纺锤体两极之间的沟。最后，我们发现只有一对中心体的无核细胞仍然试图分裂。因此，在C.在秀丽隐杆线虫胚胎细胞中，如棘皮动物中，两个紫苑的并列足以诱导犁沟，并且染色质和中央纺锤体的物理结构对于犁沟的起始都不是必不可少的。然而，与纺锤体交叉的犁沟比不与纺锤体交叉的犁沟更容易完成。
Classical experimental studies on echinoderm zygotes concluded that the juxtaposition of two astral microtubule arrays localizes the stimulus for cytokinetic furrowing. However, recent experimental and genetic studies in Caenorhabditis elegans, Drosophila and mammalian cultured cells implicate microtubules of the central spindle, and regulatory proteins associated with this structure, suggesting that the essential conditions for furrow induction may differ from one animal cell to another. We used micromanipulation and laser microsurgery to create, in three ways, the juxtaposition of astral microtubules in C. elegans embryonic cells. In toroidal cells we observe that furrows initiate both where astral microtubule arrays are juxtaposed, and where the cortex most closely approaches the central spindle. We find that binucleate cells successfully furrow not only across the spindles, but also between unconnected spindle poles. Finally, we find that anucleate cells containing only a pair of centrosomes nevertheless attempt to cleave. Therefore, in C. elegans embryonic cells, as in echinoderms, juxtaposition of two asters suffices to induce furrowing, and neither the chromatin nor the physical structure of the central spindle are indispensable for furrow initiation. However, furrows that cross a central spindle are more likely to complete than those that do not.