Mismatches improve the performance of strand-displacement nucleic Acid circuits.
Mismatches improve the performance of strand-displacement nucleic Acid circuits.
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DOI：
10.1002/anie.201307418
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发表时间：
2014-02-10
影响因子：
16.6
通讯作者：
Ellington, Andrew D
中科院分区：
文献类型：
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作者：
Jiang, Yu Sherry;Bhadra, Sanchita;Li, Bingling;Ellington, Andrew D
催化发夹组装 (CHA) 先前已被证明可用作核酸检测的转导和扩增方法。然而，即使在没有单链催化剂的情况下，CHA 电路中的两个发夹底物也可能发生非特异性反应，并且这种非特异性背景会降低信噪比。阻碍非催化链交换反应的错配碱基对的引入大大降低了背景信号，而在催化剂存在下仅部分抑制信号。通过荧光测定法对各种类型和长度的错配进行了分析，在许多情况下，我们的 MismatCHA 设计产生了 100 倍的信号背景比，而完美匹配的底物的类似比率为 4。这些观察结果可能被证明对于非酶核酸电路的设计具有普遍实用性。
Catalytic hairpin assembly (CHA) has previously proven useful as a transduction and amplification method for nucleic acid detection. However, the two hairpin substrates in a CHA circuit can potentially react non-specifically even in the absence of a singles-stranded catalyst, and this non-specific background degrades signal-to-noise. The introduction of mismatched base-pairs that impede uncatalyzed strand exchange reactions greatly decreased background signal while only partially damping signal in the presence of catalyst. Various types and lengths of mismatches were assayed by fluorimetry and in many instances our MismatCHA designs yielded 100-fold signal-to-background ratios compared to a similar ratio of 4 with the perfectly matched substrates. These observations may prove to be of general utility for the design of non-enzymatic nucleic acid circuits.