Nanaomycin A methyl ester, an actinomycete metabolite: Algicidal activity and the physiological response of Microcystis aeruginosa
Nanaomycin A methyl ester, an actinomycete metabolite: Algicidal activity and the physiological response of Microcystis aeruginosa
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纳霉素 A 甲酯，一种放线菌代谢物：铜绿微囊藻的杀藻活性和生理反应
DOI：
10.1016/j.ecoleng.2012.12.066
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发表时间：
2013-04-01
影响因子：
3.8
通讯作者：
Jiang, Yi
中科院分区：
文献类型：
--
作者：
Feng, Yang;Chang, Xuexiu;Jiang, Yi
有害蓝藻水华及其对水生态系统和人类健康的负面影响在世界各地均有报道，铜绿微囊藻是中国滇池和太湖等富营养化湖泊中最具优势且对生态破坏最严重的物种之一。虽然在短期内对水华进行物理或化学控制是可行的，但这些方法可能会引发其他问题。因此，仍有必要对控制蓝藻水华的生物因素进行研究。我们筛选了云南大学放线菌菌株库的一部分，发现河北链霉菌YIM 001(T)的提取物对铜绿微囊藻表现出明显的抑制作用。通过扩大发酵，以20mg/L的水平分离出主要成分——那那霉素A甲酯（NAME）。NAME对铜绿微囊藻的裂解机制实验表明，它能有效抑制铜绿微囊藻的生长（72小时的半数有效浓度EC50 = 2.97mg/L），阻碍细胞分裂并增大细胞尺寸。NAME对铜绿微囊藻细胞造成损伤，表现为藻类活性、生物量、酯酶活性和叶绿素 - a含量降低。处理后的细胞超氧化物歧化酶（SOD）活性先升高后降低，同时超氧阴离子（O - 2(-)）和丙二醛（MDA）含量逐渐增加，这与NAME对藻类细胞膜脂质过氧化损伤导致的抑制作用相符。结果表明，NAME对控制有害蓝藻是有效的，叶绿素荧光是检测NAME对蓝藻生态毒性的一个潜在指标。（C）2012爱思唯尔有限公司。版权所有。
Harmful cyanobacterial blooms and their negative impacts on water ecosystems and human health have been reported all over the world, and Microcystis aeruginosa is one of the most dominant and ecologically damaging species in eutrophic lakes such as Dianchi Lake and Taihu Lake in China. Although physical or chemical control of the blooms is possible in short period, these methods could cause other problems. Thus, there is still a need for research on biological factors controlling cyanobacteria blooms. We screened part of the Yunnan University actinomycete strain library and found that the extract of Streptomyces hebeiensis YIM 001(T) exhibited obvious inhibition on M. aeruginosa. The main component - nanaomycin A methyl ester (NAME) was isolated at a level of 20 mg L-1 by scale-up fermentation. Lytic mechanism experiments of NAME on M. aeruginosa demonstrated it could effectively inhibit the growth of M. aeruginosa (EC50 at 72 h = 2.97 mg L-1), hinder cell division and enlarge cell size. NAME damaged M. aeruginosa cells as evidenced by decreases in algal activity, biomass, esterase activity and chlorophyll-a content. The SOD activity of the treated cells initially increased, and then decreased together with a gradual increase in both O-2(-) and malondialdehyde (MDA) content, consistent with inhibitory effects of NAME due to lipid peroxidation damage of the algal cell membrane. The results suggest that NAME is effective for controlling undesired cyanobacteria and the Chl-fluorescence is a potential endpoint to assay the NAME eco-toxicity on cyanobacteria. (C) 2012 Elsevier B.V. All rights reserved.