HyPRP1 Gene Suppressed by Multiple Stresses Plays a Negative Role in Abiotic Stress Tolerance in Tomato.
HyPRP1 Gene Suppressed by Multiple Stresses Plays a Negative Role in Abiotic Stress Tolerance in Tomato.
复制标题
多重胁迫抑制的 HyPRP1 基因在番茄非生物胁迫耐受性中发挥负面作用。
DOI：
10.3389/fpls.2016.00967
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发表时间：
2016
影响因子：
5.6
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Li J;Ouyang B;Wang T;Luo Z;Yang C;Li H;Sima W;Zhang J;Ye Z
许多杂交富含脯氨酸蛋白（HyPRP）基因在植物中响应生物和非生物胁迫，但很少有人知道他们的角色以外的推定细胞壁结构蛋白。HyPRP 1基因编码一个富含脯氨酸结构域的蛋白质，并从我们以前的耐旱番茄微阵列实验中鉴定出一个8-半胱氨酸基序。在这项研究中，HyPRP 1基因在番茄中的表达受到抑制，在各种非生物胁迫下，如干旱，高盐，寒冷，炎热和氧化胁迫。转基因功能分析表明，表型没有明显变化，但增强了对各种非生物胁迫的耐受性（例如，氧化胁迫、脱水和盐度）。有趣的是，几个SO2解毒相关的酶，包括亚硫酸盐氧化酶，铁氧化还原酶（Fds），甲硫氨酸亚砜还原酶A（Msr A），发现在HyPRP 1相互作用的蛋白质鉴定酵母双杂交筛选。当野生型植物暴露于SO2气体中时，更多的硫酸盐和Msr A和Fds的转录本在HyPRP 1敲除系中积累。我们的研究结果表明，番茄HyPRP 1是盐和氧化应激的负调节剂，可能参与亚硫酸盐代谢。
Many hybrid proline-rich protein (HyPRP) genes respond to biotic and abiotic stresses in plants, but little is known about their roles other than as putative cell-wall structural proteins. A HyPRP1 gene encodes a protein with proline-rich domain, and an eight-cysteine motif was identified from our previous microarray experiments on drought-tolerant tomato. In this study, the expression of the HyPRP1 gene in tomato was suppressed under various abiotic stresses, such as drought, high salinity, cold, heat, and oxidative stress. Transgenic functional analysis showed no obvious changes in phenotypes, but enhanced tolerance to various abiotic stresses (e.g., oxidative stress, dehydration, and salinity) was observed in RNAi transgenic plants. Interestingly, several SO2 detoxification-related enzymes, including sulfite oxidase, ferredoxins (Fds), and methionine sulfoxide reductase A (Msr A), were revealed in HyPRP1-interacting proteins identified by Yeast Two-Hybrid screening. More sulfates and transcripts of Msr A and Fds were accumulated in HyPRP1 knockdown lines when wild-type plants were exposed to SO2 gas. Our findings illustrate that the tomato HyPRP1 is a negative regulator of salt and oxidative stresses and is probably involved in sulfite metabolism.