Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions
Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions
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DOI：
10.1105/tpc.12.5.721
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发表时间：
2000-05-01
期刊：
影响因子：
11.6
通讯作者：
Nawrath, C
中科院分区：
文献类型：
--
作者：
Sieber, P;Schorderet, M;Nawrath, C
植物角质层的主要结构组分是角质，由于没有表征具有角质特异性缺陷的突变体，因此对角质在体内的功能的分析受到限制。因此，产生了表达和分泌来自梨腐镰刀菌豌豆专化型的角质酶的转基因拟南芥植物。在细胞外空间表达角质酶的拟南芥植株表现出角质层超微结构的改变和角质层对溶质的渗透性增强。此外，花粉可以在角质酶表达植物的完全分化的叶片上萌发，但不能在对照叶片上萌发。这些差异与强烈的后生殖器官融合一致。融合体的连接处含有果胶多糖。随着融合器官彼此分开生长，器官变形和表皮细胞突起在具有高机械应力的位置处发展。这些结果表明，一个完整的角质层不仅是重要的植物与环境的相互作用，但也防止不同植物器官之间的融合，因此是必要的正常表皮分化和器官形成。
A major structural component of the cuticle of plants is cutin, Analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. Therefore, transgenic Arabidopsis plants were generated that express and secrete a cutinase from Fusarium solani f sp pisi. Arabidopsis plants expressing the cutinase in the extracellular space showed an altered ultrastructure of the cuticle and an enhanced permeability of the cuticle to solutes. In addition, pollen could germinate on fully differentiated leaves of cutinase-expressing plants but not on control leaves. These differences coincided with strong postgenital organ fusions. The junctions of the fusions contained pectic polysaccharides. As fused organs grew apart from each other, organ deformations and protrusions of epidermal cells developed at positions with high mechanical stress. These results demonstrate that an intact cutin layer not only is important for plant-environment interactions but also prevents fusions between different plant organs and is therefore necessary for normal epidermal differentiation and organ formation.