Silver binding to rabbit liver zinc metallothionein and zinc .alpha. and .beta. fragments. Formation of silver metallothionein with silver(I):protein ratios of 6, 12, and 18 observed using circular dichroism spectroscopy
Silver binding to rabbit liver zinc metallothionein and zinc .alpha. and .beta. fragments. Formation of silver metallothionein with silver(I):protein ratios of 6, 12, and 18 observed using circular dichroism spectroscopy
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DOI：
10.1021/ic00042a008
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发表时间：
1992-08
影响因子：
4.6
通讯作者：
A. Żelazowski;M. Stillman
中科院分区：
文献类型：
--
作者：
A. Żelazowski;M. Stillman
通过对用 Ag + 滴定蛋白质期间在 5 至 55 o C 之间记录的圆二色性 (CD) 光谱数据进行分析，报道了兔肝锌金属硫蛋白 (MT) 以及 Zn 3 -β MT 1 和 Zn 4 -α MT 1 片段中 Ag + 和半胱氨酸硫醇盐基团之间形成的一系列复合物。光谱包络揭示了 Ag 12 -MT、Ag 18 -MT、Ag 6 -α MT 和 Ag 6 -β MT 的形成
Formation of a series of complexes between Ag + and the cysteine thiolate groups in rabbit liver zinc metallothionein (MT) and the Zn 3 -β MT 1 and Zn 4 -α MT 1 fragments is reported from analysis of the circular dichroism (CD) spectral data recorded between 5 and 55 o C during titrations of the protein with Ag + . The spectral envelopes reveal formation of Ag 12 -MT, Ag 18 -MT, Ag 6 -α MT, and Ag 6 -β MT