Regulating cytoplasmic oxalate homeostasis by Acyl activating enzyme3 is critical for plant Al tolerance
Regulating cytoplasmic oxalate homeostasis by Acyl activating enzyme3 is critical for plant Al tolerance
复制标题
通过酰基激活酶调节细胞质草酸稳态对于植物铝耐受性至关重要
DOI：
10.1080/15592324.2016.1276688
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发表时间：
2017-01-01
影响因子：
2.9
通讯作者：
Zheng, Shao Jian
中科院分区：
文献类型：
--
作者：
Chen, Wei Wei;Fan, Wei;Zheng, Shao Jian
草酸是二元酸中最简单的。草酸除了在生物和代谢过程中发挥作用外，还与生物和非生物胁迫有关。草酸盐作为铝的强络合剂，在铝抗性机制中也起着关键作用。然而，我们证明了细胞质草酸积累是导致铝胁迫下根生长抑制的关键事件。对三种作物的转录组分析发现，AAE3基因在铝胁迫下表达上调。这些AAE3蛋白与已知的AAE3蛋白显示出高度的序列相似性，表明它们是草酸合成酶，特别参与草酸的降解。然而，系统发育分析揭示了AAE3在单子叶植物和双子叶植物之间的差异，这表明其他植物的AAE3蛋白在铝胁迫下的功能特征是必要的。
Oxalic acid is the simplest of the dicarboxylic acids. In addition to its role in biological and metabolic processes, oxalate has been implicated in biotic and abiotic stresses. Being a strong chelator of Al, oxalate also has pivotal role in Al resistance mechanisms. However, we demonstrated that cytoplasmic oxalate accumulation is a critical event leading to root growth inhibition under Al stress. Transcriptome analysis from three crop plants identified Acyl Activating Enzyme3 (AAE3) genes to be upregulated by Al stress. These AAE3 proteins display high sequence identity to known AAE3 proteins, suggesting they are oxalylCo-A synthetases specifically involved in oxalate degradation. However, phylogenetic analysis revealed divergence of AAE3 between monocots and dicots, pointing to the necessity for functional characterization of AAE3 proteins from other plant species with respect to Al stress.