Autophagy-mediated stress response in motor neurons after hypothermic spinal cord ischemia in rabbits
Autophagy-mediated stress response in motor neurons after hypothermic spinal cord ischemia in rabbits
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DOI：
10.1016/j.jvs.2014.03.297
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发表时间：
2015-11-01
影响因子：
4.3
通讯作者：
Tominaga, Ryuji
中科院分区：
文献类型：
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作者：
Fujita, Satoshi;Sakurai, Masahiro;Tominaga, Ryuji
目的：脊髓损伤的发生发展与脊髓运动神经元对缺血的易损性有关。然而，这种脆弱性的机制尚未得到充分调查。以前，我们报道了脊髓运动神经元可能由于自噬而丢失，局部低温可防止这种脊髓运动神经元死亡。因此，我们使用Beclin 1的免疫组织化学分析来研究自噬在常温和低温脊髓缺血中的作用。（BCLN 1; B细胞白血病2蛋白[Bcl-2]相互作用蛋白）、Bcl-2和g-氨基丁酸A型受体相关蛋白（GABARAP），它们被认为是自噬相关蛋白。我们使用兔常温和低温短暂性脊髓缺血模型，使用球囊导管。根据约翰逊评分评估神经功能，并在再灌注后8 h和1、2、7 d取出脊髓，苏木精-伊红染色观察形态学变化。结果：低温组与常温组相比，低温组的生理功能有显著性差异（P
Objective: The development of spinal cord injury is believed to be related to the vulnerability of spinal motor neurons to ischemia. However, the mechanisms underlying this vulnerability have not been fully investigated. Previously, we reported that spinal motor neurons are lost likely due to autophagy and that local hypothermia prevents such spinal motor neuron death. Therefore, we investigated the role of autophagy in normothermic and hypothermic spinal cord ischemia using an immunohistochemical analysis of Beclin 1 (BCLN1; B-cell leukemia 2 protein [Bcl-2] interacting protein), Bcl-2, and g-aminobutyric acid type-A receptor-associated protein (GABARAP), which are considered autophagy-related proteins.Methods: We used rabbit normothermic and hypothermic transient spinal cord ischemia models using a balloon catheter. Neurologic function was assessed according to the Johnson score, and the spinal cord was removed at 8 hours and 1, 2, and 7 days after reperfusion, and morphologic changes were examined using hematoxylin and eosin staining. A Western blot analysis and histochemical study of BCLN1, Bcl-2, and GABARAP, and double-labeled fluorescent immunocytochemical studies were performed.Results: There were significant differences in the physiologic function between the normothermic model and hypothermic model after the procedure (P