Induction of cyclooxygenase-2 expression by interleukin-1beta in human glioma cell line, U87MG.
Induction of cyclooxygenase-2 expression by interleukin-1beta in human glioma cell line, U87MG.
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DOI：
10.2176/nmc.48.500
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发表时间：
2008
影响因子：
1.9
通讯作者：
S. Taniura;H. Kamitani;Takashi Watanabe;T. Eling
中科院分区：
文献类型：
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作者：
S. Taniura;H. Kamitani;Takashi Watanabe;T. Eling
环氧化酶-2 （COX-2）在大多数高级别胶质瘤中上调，高COX-2表达与侵袭性和不良预后相关。然而，COX-2在人类胶质瘤细胞系中的作用尚不清楚。本研究检测了几种刺激物，包括白细胞介素-1 β (il -1 β)和致癌物，对正常星形胶质细胞和人类胶质瘤细胞系U87MG、A172和T98G中COX-2诱导的影响。il -1 β仅在胶质瘤细胞系的U87MG细胞中诱导COX-2在蛋白和信使核糖核酸水平上的强烈表达。此外，致癌物诱导COX-2表达。在正常的人类星形胶质细胞中也观察到类似的结果。U87MG胶质瘤细胞系是COX-2诱导胶质瘤细胞系的良好模型。
Cyclooxygenase-2 (COX-2) is up-regulated in most high-grade gliomas, and high COX-2 expression is associated with aggressive character and poor prognosis. However, the effect of COX-2 in human glioma cell lines is not well known. This study examined the effect of several stimuli, including interleukin-1beta (IL-1beta) and carcinogens, on COX-2 induction in normal astrocyte cells and human glioma cell lines U87MG, A172, and T98G. IL-1beta-induced COX-2 expression strongly at both protein and messenger ribonucleic acid levels in only the U87MG cells of the glioma cell lines. Furthermore, carcinogen induced COX-2 expression. Similar findings were also observed in normal human astrocyte cells. The U87MG glioma cell line is a good model for COX-2 induction in glioma cell lines.