Nucleocytoplasmic Traffic of CPEB1 and Accumulation in Crm1 Nucleolar Bodies
Nucleocytoplasmic Traffic of CPEB1 and Accumulation in Crm1 Nucleolar Bodies
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DOI：
10.1091/mbc.e08-09-0904
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Weil, Dominique
中科院分区：
文献类型：
--
作者：
Ernoult-Lange, Michele;Wilczynska, Ania;Weil, Dominique
翻译调节因子CPEB 1在卵母细胞中母体mRNA的控制以及神经元中突触下mRNA的控制中起主要作用。虽然主要是细胞质，我们发现CPEB 1蛋白是不断穿梭于细胞核和细胞质之间。它的输出由依赖于核输出受体Crm1的两个冗余内斯基序控制。在细胞核中，CPEB 1聚集在少数病灶中，最常与核仁相关。这些病灶与以前发现的核小体不同。它们含有Crm1，被称为Crm1核仁体（CNoBs）。CNoBs依赖于RNA聚合酶I活性，表明在核糖体生物合成中的作用。然而，尽管它们在核仁中形成，但它们从不迁移到核膜，排除了作为核糖体输出的介导剂的作用。相反，它们可以构成一个平台，为核糖体组装或输出提供因子。CPEB1在CNoBs中的行为提出了它参与核糖体生物合成的可能性。
The translational regulator CPEB1 plays a major role in the control of maternal mRNA in oocytes, as well as of subsynaptic mRNAs in neurons. Although mainly cytoplasmic, we found that CPEB1 protein is continuously shuttling between nucleus and cytoplasm. Its export is controlled by two redundant NES motifs dependent on the nuclear export receptor Crm1. In the nucleus, CPEB1 accumulates in a few foci most often associated with nucleoli. These foci are different from previously identified nuclear bodies. They contain Crm1 and were called Crm1 nucleolar bodies (CNoBs). CNoBs depend on RNA polymerase I activity, indicating a role in ribosome biogenesis. However, although they form in the nucleolus, they never migrate to the nuclear envelope, precluding a role as a mediator for ribosome export. They could rather constitute a platform providing factors for ribosome assembly or export. The behavior of CPEB1 in CNoBs raises the possibility that it is involved in ribosome biogenesis.