Antigens of Treponema pallidum recognized by IgG and IgM antibodies during syphilis in humans.
Antigens of Treponema pallidum recognized by IgG and IgM antibodies during syphilis in humans.
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人类梅毒期间 IgG 和 IgM 抗体识别的梅毒螺旋体抗原。
DOI：
10.1093/infdis/151.2.264
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Lukehart,SA
中科院分区：
文献类型：
--
作者：
Baker-Zander,SA;Hook3rd,EW;Bonin,P;Handsfield,HH;Lukehart,SA
本文应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹技术检测了梅毒初治患者、正常人、梅毒生物学假阳性者和梅毒感染者性接触者血清中梅毒螺旋体Nichols株抗原的IgG和IgM抗体。一期梅毒患者血清的IgG反应性差异很大，但始终表现出强烈的反应性48千道尔顿带。从患者的血清与二期和早期潜伏梅毒一致表现出反应性22个独立的多肽抗原，反应性下降，在晚期潜伏梅毒。正常和生物假阳性血清表现出弱的IgG反应性对没有到12多肽。感染性梅毒患者的无症状接触者的血清对不同数量的密螺旋体抗原表现出反应性，包括一些正常血清所未见的反应。IgM反应是最突出的二期梅毒，但在疾病的各个阶段都是显而易见的。
IgG and IgM antibody specificities for antigens ofTreponema pallidumNichols strain were determined by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the western blot technique in sera from patients with untreated syphilis, normal persons, persons with biologic false-positive tests for syphilis, and sexual contacts of persons with infectious syphilis. IgG reactivities of sera from individuals with primary syphilis varied considerably but consistently exhibited strong reactivity to a 48-kilodalton band. Sera from patients with secondary and early latent syphilis uniformly demonstrated reactivity to 22 separate polypeptide antigens; decreased reactivity was seen in late latent syphilis. Normal and biologic false-positive sera showed weak IgG reactivity against none to 12 polypeptides. Sera from asymptomatic contacts of persons with infectious syphilis showed reactivity to a varying number of treponemal antigens, including some reactions not seen with normal sera. IgM reactivity was most prominent in secondary syphilis but was demonstrable at all stages of disease.