KARYOTYPE CONSISTENCY IN HUMAN COLORECTAL-CARCINOMA CELL-LINES ESTABLISHED INVITRO
KARYOTYPE CONSISTENCY IN HUMAN COLORECTAL-CARCINOMA CELL-LINES ESTABLISHED INVITRO
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DOI：
10.1016/0165-4608(82)90076-0
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发表时间：
1982-01-01
影响因子：
--
通讯作者：
MACY, ML
中科院分区：
文献类型：
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作者：
CHEN, TR;HAY, RJ;MACY, ML
通过胰蛋白酶-吉姆萨显带法对保藏于 ATCC [美国典型培养物保藏中心] 的 9 个人类结直肠细胞系的核型进行了研究。 CCL 229、230、231和235（众数染色体数Sm分别为49、68、47和40）属于稳定型，其核型主要由正常染色体和稳定标记组成。 CCL 228、234和238（Sm分别为55、79和70）属于不稳定型，其核型除正常染色体和稳定标记外还包含大量标记。其余的中间类型（CCL 233 和 237，Sm 分别为 60 和 64）具有介于上述 2 种类型之间的核型特征，通常每个细胞具有 2 个或更少的不稳定标记。稳定标记（与正常染色体一起）是细胞基因组的组成部分，并且对于同一培养群体中的大多数细胞来说是常见的。不稳定标记通常仅构成总染色体补体的一小部分，是细胞间核型变异的可能过程，并且通常是由平衡的染色体间或染色体内变化或两者兼而有之产生的。每个细胞基因组的总染色体长度在细胞群内非常一致，并且细胞之间的核型（例如来自 4 个稳定系的细胞）非常稳定并且大部分相同。在稳定和不稳定标记中，染色体缺失和同系物间交换（包括同染色体）发生率最高。复杂标记出现的频率相对较低。这些既不是所有这些已建立的细胞系共有的共同标记，也不是独特的染色体断裂。 7 号和 1 号染色体的结构修饰发生率最高（分别为 15 次和 12 次），导致形成稳定标记（9 个细胞系中总共 82 次交换），7 号和 2 号染色体参与稳定和不稳定标记形成的高发生率（分别为 21 次和 15 次）（总共 181 次交换）。 7 号染色体在 9 条线中的 8 条中比例过高。简要讨论了涉及该肿瘤以及其他肿瘤病理型的 7 号染色体变化的重要性。
Karyotypes of 9 human colorectal cell lines deposited with the ATCC [American Type Culture Collection] were studied by trypsin-Giemsa banding. CCL 229, 230, 231 and 235 (Modal chromosome number, Sm, was 49, 68, 47 and 40, respectively) belong in the stable type that is characterized by karyotypes consisting mostly of normal chromosomes and stable markers. CCL 228, 234 and 238 (Sm = 55, 79 and 70, respectively) belong in the unstable type that has karyotypes consisting of numerous markers in addition to normal chromosomes and stable markers. The remaining intermediate type (CCL 233 and 237, Sm = 60 and 64, respectively) has karyotypic characteristics between the above 2 types usually with 2 or less unstable markers per cell. The stable markers (together with normal chromosomes) are constitutive components of a cell genome and are common to most cells within the same cultured population. Unstable markers, which generally constitute only a small portion of the total chromosome complement are the likely course of karyotypic variations between cells and often are produced by balanced inter- or intrachromosome changes, or both. Total chromosome length per cell genome is remarkably consistent within a cell population, and karyotypes between cells, such as from 4 stable lines, are profoundly stable and mostly identical. Chromosome deletions and interhomologue exchanges (including isochromosomes) had the highest incidences among stable and unstable markers. The complex markers occurred relatively infrequently. These were neither common markers nor unique chromosome breakages common to all of these established cell lines. Chromosomes 7 and 1 had the highest incidence (15 and 12, respectively) of structural modifications resulting in the formation of stable markers (82 total exchanges in 9 cell lines), and chromosomes 7 and 2 were involved at high incidence (21 and 15, respectively) in the formation of stable and unstable markers (181 total exchanges). Chromosome 7 is overrepresented in 8 of 9 lines. The significance of chromosome changes involving 7 in this as well as other tumor pathotypes is briefly discussed.