Novaeguinoside II inhibits cell proliferation and induces apoptosis of human brain glioblastoma U87MG cells through the mitochondrial pathway
Novaeguinoside II inhibits cell proliferation and induces apoptosis of human brain glioblastoma U87MG cells through the mitochondrial pathway
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DOI：
10.1016/j.brainres.2010.11.093
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发表时间：
2011-02
期刊：
影响因子：
2.9
通讯作者：
Jie Zhou;Gang Cheng;G. Cheng;Hai-Feng Tang;Xiang Zhang
中科院分区：
文献类型：
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作者：
Jie Zhou;Gang Cheng;G. Cheng;Hai-Feng Tang;Xiang Zhang
Novaeguinoside II是新发现的海星皂苷之一，是从海星中提取的具有抗肿瘤作用的甾体苷类化合物。在这项研究中，我们研究了Novaeguinoside II对人U87 MG胶质母细胞瘤细胞的生物活性。结果表明，Novaeguinoside II显著抑制U87 MG细胞增殖，并呈时间和浓度依赖性。U87 MG细胞中DNA的流式细胞术分析显示，Novaeguinoside II诱导细胞周期中S期峰的显著出现，这提示细胞凋亡，这与膜联蛋白V/PI测定的结果相同。荧光显微镜和电镜观察显示U87 MG细胞凋亡改变。DNA电泳显示典型的“梯状”，与凋亡DNA片段化一致。细胞荧光分析显示，降低线粒体跨膜电位在Novaeguinoside II处理的U87 MG细胞。Western blot结果显示，Novaeguinoside II可增加细胞色素C和caspase-3蛋白的表达。新的发现表明，Novaeguinoside II可以通过线粒体凋亡途径诱导U87 MG细胞凋亡。
Novaeguinoside II is one of the newly found asterosaponins, which are a kind of steroidal glycosides extracted from starfish with an anti-tumor effect. In this study, we investigated the bioactivity of novaeguinoside II on human U87MG glioblastoma cells. The results showed that novaeguinoside II significantly suppresses U87MG cell proliferation in a time- and concentration-dependent manner. Flow cytometric analysis of DNA in U87MG cells showed that novaeguinoside II induces the prominent appearance of an S phase peak in the cell cycle that is suggestive of apoptosis that is identical to the result of an annexin V/PI assay. Fluorescence and electron microscopy revealed apoptotic change of U87MG cells. The electrophoresis of DNA showed a typical “ladder” that is consistent with apoptotic DNA fragmentation. Cytofluorometry showed a decreased mitochondrial transmembrane potential in novaeguinoside II-treated U87MG cells. Western blot showed that novaeguinoside II increased the expression of cytochrome-c and caspase-3 protein. The novel findings suggest that novaeguinoside II can induce apoptosis of U87MG cells by a mitochondrial apoptotic pathway.