Distinct mammalian precursors are committed to generate neurons with defined dendritic projection patterns
Distinct mammalian precursors are committed to generate neurons with defined dendritic projection patterns
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DOI：
10.1371/journal.pbio.0050300
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发表时间：
2007-11-01
期刊：
影响因子：
9.8
通讯作者：
Lois, Carlos
中科院分区：
文献类型：
--
作者：
Kelsch, Wolfgang;Mosley, Colleen P.;Lois, Carlos
调节树突如何靶向不同神经元以与特定细胞类型建立连接的机制在很大程度上仍然未知。特别是，在出生后的神经发生过程中，细胞类型特异性连接的形成可以由成熟神经元回路的局部环境决定，也可以由未成熟神经元的细胞自主特性决定，这些特性已经由它们的前体决定。使用逆转录病毒的命运映射，我们研究了一种神经元类型的板层特异性树突状靶向定义其形态和内在的体细胞电特性在新生儿和成人神经发生。命运映射显示存在两个独立的群体的神经元前体，产生了相同的神经元类型与两种不同的模式的树突靶向神经支配的深层或浅层，在那里他们连接到不同类型的主神经元。此外，异时和异位移植表明，这些前体在很大程度上被限制为生成具有预定模式的树突靶向的神经元，该模式独立于宿主环境。我们的研究结果表明，至少在新生儿和成年哺乳动物的大脑中，树突靶向给定的神经元的模式是其前体细胞的自主性质。
The mechanisms that regulate how dendrites target different neurons to establish connections with specific cell types remain largely unknown. In particular, the formation of cell-type-specific connectivity during postnatal neurogenesis could be either determined by the local environment of the mature neuronal circuit or by cell-autonomous properties of the immature neurons, already determined by their precursors. Using retroviral fate mapping, we studied the lamina-specific dendritic targeting of one neuronal type as defined by its morphology and intrinsic somatic electrical properties in neonatal and adult neurogenesis. Fate mapping revealed the existence of two separate populations of neuronal precursors that gave rise to the same neuronal type with two distinct patterns of dendritic targeting-innervating either a deep or superficial lamina, where they connect to different types of principal neurons. Furthermore, heterochronic and heterotopic transplantation demonstrated that these precursors were largely restricted to generate neurons with a predetermined pattern of dendritic targeting that was independent of the host environment. Our results demonstrate that, at least in the neonatal and adult mammalian brain, the pattern of dendritic targeting of a given neuron is a cell-autonomous property of their precursors.