Real-time PCR to improve the diagnosis of respiratory syncytial virus infection
Real-time PCR to improve the diagnosis of respiratory syncytial virus infection
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DOI：
10.1099/jmm.0.05290-0
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发表时间：
2003-10-01
影响因子：
3
通讯作者：
G端rtler, L
中科院分区：
文献类型：
--
作者：
Mentel, R;Wegner, U;G端rtler, L
呼吸道合胞病毒（Respiratory syncytial virus，RSV）是婴幼儿呼吸道最重要的病毒病原之一。快速和敏感的诊断是必不可少的，以集中任何爆发，由于这种病毒。使用来自F基因的引物/探针对设计实时RT-PCR方法。同时应用巢式RT-PCR和抗原ELISA检测了71例急性呼吸窘迫住院患儿的RSV感染情况，实时荧光定量RT-PCR检测25例（35.2%），巢式RT-PCR检测19例（26.7%）。该试验对RSV具有特异性。该方法提供了一种快速和灵敏的替代传统的RT-PCR。闭管检测消除了污染的风险。
Respiratory syncytial virus (RSV) is one of the most important virus respiratory pathogens in infants and young children. A rapid and sensitive diagnosis is essential to focus any outbreak due to this virus. A real-time RT-PCR method was designed using a primer/probe pair from the F gene. Simultaneously with nested RT-PCR and antigen ELISA, 71 consecutive specimens from hospitalized children with clinical symptoms of acute respiratory distress were evaluated to confirm the incidence of RSV infection, RSV was detected in 25 (35.2 %) specimens by real-time RT-PCR and in 19 (26.7 %) by nested RT-PCR. The assay was specific for RSV. The procedure offers a rapid and sensitive alternative to conventional RT-PCR. Closed-tube detection eliminates the risk of contamination.