Facile Gene Delivery Derived from Branched Low Molecular Weight Polyethylenimine by High Efficient Chemistry.
Facile Gene Delivery Derived from Branched Low Molecular Weight Polyethylenimine by High Efficient Chemistry.
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DOI：
10.1166/jbn.2018.2620
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发表时间：
2018-10-01
影响因子：
2.9
通讯作者：
Dou H
中科院分区：
文献类型：
--
作者：
Zou L;Lee SY;Wu Q;Zhang H;Bastian A;Orji C;Payne G;Galvez A;Thomas T;Zhang Z;Dou H
介绍了基于支链低分子量聚乙烯亚胺（PEI）制备基因传递平台的简便方法。首先，利用PEI中的胺与缩水甘油酯十六烷基醚（环氧c16）进行高效开环反应，制备出具有低分子量PEI头基和十六烷基链尾基的PEI脂质。然后，将PEI脂质作为阳离子脂质体的组成部分，并作为表面活性剂，采用溶剂萃取/蒸发法制备聚D， l -丙交酯-羟基乙酸酯（PLGA）纳米颗粒（NP）。作为潜在的有效基因传递平台，研究了它们的制备、大小、大小分布、毒性、质粒DNA装载、体外转染和细胞内运输。与高分子量PEI相比，这两种简易平台在体外均表现出更低的毒性和更高的转染效率，在基因传递领域可能有进一步的广泛应用。
Convenient methods for the preparation of gene delivery platforms based on branched low molecular weight polyethylenimine (PEI) were described. Firstly, PEI lipids, with a low molecular weight PEI headgroup and hexadecyl chain tail group, were prepared through a highly efficient ring-opening reaction of glycidyl hexadecyl ether (EpoxyC16) by amine from PEI. Then, the PEI lipids were used as a component of cationic liposomes and as a surfactant for the preparation of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticle (NP) via solvent extraction/evaporation method. As potential effective gene delivery platforms, their preparation, size, size distribution, toxicities, plasmid DNA loading, in vitro transfection and intracellular trafficking were studied. Both facile platforms showed less toxicity and higher transfection efficacy when compared to high molecular weight PEI in vitro, and may have further versatile applications in the gene delivery field.
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影响因子：
10.8
作者：
Kleemann, E;Neu, M;Kissel, T
通讯作者：
Kissel, T
影响因子：
14
作者：
Cheng, Wei;Yang, Chuan;Ashton-Rickardt, Philip G.
通讯作者：
Ashton-Rickardt, Philip G.
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影响因子：
6.2
作者：
Chen, Jing;Liu, Qiuming;Du, Jianzhong
通讯作者：
Du, Jianzhong
影响因子：
14
作者：
Kim, Ji-Young;Kim, Jin-Ki;Kim, Chong-Kook
通讯作者：
Kim, Chong-Kook
影响因子：
2.9
作者：
Duan, Siliang;Song, Mongkhoune;Lu, Xiaoling
通讯作者：
Lu, Xiaoling