Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast.
Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast.
复制标题
Ty 1-17（酵母的 II 类转座子）的核苷酸序列特征。
DOI：
10.1093/nar/13.18.6679
复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Oliver,SG
中科院分区：
文献类型：
--
作者：
Warmington,JR;Waring,RB;Newlon,CS;Indge,KJ;Oliver,SG
我们已经确定了 II 类酵母转座子 (Ty 1-17) 的核苷酸序列，该转座子位于酿酒酵母 III 号染色体上 LEU2 结构基因着丝粒远端。完整的元件长 5961 bp，并由两个相同的、直接重复的、各 332 bp 的 delta 序列界定。序列组织表明 Ty 1-17 是逆转录转座子，就像之前表征的 I 类元件一样。它包含两个长开放阅读框：TyA（439 个氨基酸）和 TyB（1349 个氨基酸）。在本文中，对两类酵母转座子的序列进行了相互比较，并与类似元件（例如逆转录病毒原病毒、花椰菜花叶病毒和 copia 序列）进行了比较。讨论了 Ty 1-17 序列的特征，这些特征可能对其转座机制及其遗传作用很重要。
We have determined the nucleotide sequence of a class II yeast transposon (Ty 1–17) which is found just centromere-distal to the LEU2 structural gene on chromosome III of Saccharomyces cerevisiae. The complete element is 5961 bp long and is bounded by two identical, directly repeated, delta sequences of 332 bp each. The sequence organization indicates that Ty 1–17 is a retrotransposon, like the class I elements characterized previously. It contains two long open reading-frames, TyA (439 amino acids) and TyB (1349 amino acids). In this paper, the sequences of the two classes of yeast transposon are compared with one another and with analogous elements, such as retroviral proviruses, cauliflower mosaic virus and copia sequences. Features of the Ty 1–17 sequence which may be important to its mechanism of transposition and its genetic action are discussed.