An enzyme immunoassay for the detection of human tumor nucleolar antigens.
An enzyme immunoassay for the detection of human tumor nucleolar antigens.
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用于检测人类肿瘤核仁抗原的酶免疫分析。
DOI：
10.1016/0304-3835(81)90171-3
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发表时间：
1981
期刊：
影响因子：
9.7
通讯作者：
H. Busch
中科院分区：
文献类型：
--
作者：
D. Kelsey;R. Busch;H. Busch
建立了一种酶免疫测定法（EIA），将核提取物与聚苯乙烯微滴板的孔结合。用抗HeLa核仁血清检测提取液中多种抗原的存在。正常的人类细胞提取物，偶联的Sepharose作为固相吸收剂，用于去除抗体针对抗原存在于正常细胞。所得纯化的抗体在肿瘤提取物100-500 ng范围内具有线性的EIA吸光度，但对人胎盘和肝脏的核提取物的EIA吸光度很小。
An enzyme immunoassay (EIA) was developed in which nuclear extracts were bound to the wells of polystyrene microtiter plates. The presence of various antigens in the extracts could be detected using antiserum raised against HeLa cell nucleoli. Normal human cell extracts, coupled to Sepharose as a solid phase absorbent, were used to remove antibodies directed against antigens present in normal cells. The resulting purified antibodies produced a linear EIA absorbance in the range of 100–500 ng tumor extract, but little EIA absorbance with nuclear extracts from human placenta and liver.
通过二维凝胶电泳比较几种人类肿瘤和正常细胞的核蛋白。
影响因子：
11.2
作者：
Wu,BC;Spohn,WH;Busch,H
通讯作者：
Busch,H