Fish SUMO3 functions as a critical antiviral molecule against iridovirus and nodavirus
Fish SUMO3 functions as a critical antiviral molecule against iridovirus and nodavirus
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鱼 SUMO3 作为针对虹彩病毒和诺达病毒的关键抗病毒分子
DOI：
10.1016/j.fsi.2018.12.059
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发表时间：
2019
影响因子：
4.7
通讯作者：
Qin Qiwei
中科院分区：
文献类型：
--
作者：
Wei Jingguang;Li Chen;Zhang Xin;Fan Lanfen;Wei Shina;Qin Qiwei
蛋白质SUMO化（SUMO是一种小分子泛素相关修饰物）是一个受生理和病理条件严格调控的动态过程。我们以前克隆和表征两个相扑同源基因（EcSUMO 1和EcSUMO 2）从橙点石斑鱼（Epineoparus coioides）。在本研究中，SUMO 3同源物弗罗姆.克隆了EcSUMO 3基因，并分析了其在鱼类免疫中的可能作用。EcSUMO 3的开放阅读框包含285个碱基对，编码94个氨基酸，预测分子量为10.73 kDa。EcSUMO 3蛋白序列与哺乳动物相似，包括UBQ结构域、疏水表面、Ulp 1-Smt 3相互作用位点、VKTE基序和C端Gly残基。EcSUMO 3与EcSUMO 1和EcSUMO 2的同源性分别为46.83%和89.58%，与尼罗罗非鱼、斑马鱼和智人的SUMO 3的同源性分别为94%、98%和98%。实时定量PCR分析表明EcSUMO 3在健康石斑鱼的所有组织中均组成型表达。石斑鱼脾（GS）细胞中EcSUMO 3的表达水平在红斑石斑鱼神经坏死病毒（RGNNV）和新加坡石斑鱼虹彩病毒（SGIV）的刺激下显著上调（p < 0.01）。EcSUMO 3在GS细胞中既存在于细胞质中，也存在于细胞核中。EcSUMO 3在体外增强SGIV和RGNNV的复制。这些结果对于更好地理解鱼类SUMO通路和深入了解病毒感染的调控机制具有重要意义。在养殖条件下。
Protein SUMOylation (SUMO is small ubiquitin-related modifier) is a dynamic process that is strictly regulated under physiological and pathological conditions. We previously cloned and characterized two SUMO homologue genes (EcSUMO1 and EcSUMO2) from orange-spotted grouper (Epinephelus coioides). In the present study, the SUMO3 homologue fromE. coioides(EcSUMO3) was cloned and its possible roles in fish immunity were analyzed. The open reading frame of EcSUMO3 contains 285 base pairs encoding a 94 amino acid protein with a predicted molecular mass of 10.73 kDa. The protein sequence of EcSUMO3 revealed similar domains with mammals, including the UBQ (ubiquitin-like proteins) domain, the hydrophobic surface, the Ulp1-Smt3 interaction sites, a VKTE motif and the C-terminal Gly residues. EcSUMO3 shares 46.83% and 89.58% identity with EcSUMO1 and EcSUMO2, respectively, and it shares 94%, 98%, and 98% identity with SUMO3 from Oreochromis niloticus,Danio rerio, and Homo sapiens, respectively. Quantitative real-time polymerase chain reaction analysis indicated that EcSUMO3 was constitutively expressed in all of the analyzed tissues in healthy grouper. EcSUMO3 expression levels were remarkably (p < 0.01) up-regulated in grouper spleen (GS) cells in response to stimulation with red-spotted grouper nervous necrosis virus (RGNNV) and Singapore grouper iridovirus (SGIV). EcSUMO3 was distributed in both the cytoplasm and nucleus in GS cells. EcSUMO3 enhanced SGIV and RGNNV replication during viral infectionin vitro. These results are important for better understanding of the SUMO pathway in fish and provide insights into the regulatory mechanism of viral infection inE. coioidesunder farmed conditions.