Hybrids from normal, germ free, nude and neonatal mice produce monoclonal autoantibodies to eight different intracellular structures.
Hybrids from normal, germ free, nude and neonatal mice produce monoclonal autoantibodies to eight different intracellular structures.
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来自正常、无菌、裸鼠和新生小鼠的杂种可产生针对八种不同细胞内结构的单克隆自身抗体。
DOI：
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发表时间：
1985
影响因子：
4.6
通讯作者：
B. Toh
中科院分区：
文献类型：
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作者：
J. Underwood;J. Pedersen;P. Chalmers;B. Toh
将正常小鼠、无菌小鼠、裸小鼠和新生BALB/c小鼠脾细胞与小鼠NS-1骨髓瘤细胞融合产生的杂种上清液与同系小鼠成纤维细胞和小鼠3T3细胞的活细胞和丙酮固定单层以及同系小鼠红细胞和胸腺细胞的活细胞悬液进行免疫荧光反应性检测。正常小鼠的419个杂交体中有70个（16.7%），无菌小鼠的627个杂交体中有87个（13.9%），裸鼠的240个杂交体中有28个（11.7%），新生小鼠的1020个杂交体中有42个（4%）的上清液与小鼠成纤维细胞和3T3细胞的8种不同细胞内结构以及大鼠和人成纤维细胞发生反应。反应性胞内结构包括应力纤维、中间丝、相关细胞膜、高尔基复合体、细胞质、胞质囊泡、线粒体和细胞核。在45个通过限制稀释获得的稳定克隆中，43个产生IgM抗体和2个IgG2b抗体。2306个杂交株中只有一个（0.04%）产生了针对小鼠胸腺细胞表面膜的自身抗体。这些结果表明，对不同细胞内结构具有反应性的B细胞存在于正常B细胞库中，而表面反应性B细胞可能被删除或更深刻地抑制或无能，无法形成有活力的产生Ig的杂交瘤。
The supernatants of hybrids produced by fusion of splenocytes from normal, germ free, nude and neonatal BALB/c mice with mouse NS-1 myeloma cells were examined for immunofluorescence reactivity with viable and acetone fixed monolayers of syngeneic mouse fibroblasts and mouse 3T3 cells and with viable cell suspensions of syngeneic mouse erythrocytes and thymocytes. The supernatants of 70 out of 419 (16.7%) hybrids from normal mice, 87 out of 627 (13.9%) from germ free mice, 28 out of 240 (11.7%) from nude mice and 42 out of 1020 (4%) from neonatal mice reacted with 8 different intracellular structures in mouse fibroblasts and 3T3 cells, and with rat and human fibroblasts. The reactive intracellular structures comprised stress fibres, intermediate filaments, cell membrane associated, golgi complex, cytoplasm, cytoplasmic vesicles, mitochondria and nuclei. Of 45 stable clones derived by limiting dilution, 43 produced IgM antibodies and two IgG2b antibodies. Only one out of the 2,306 (0.04%) hybrids produced an autoantibody to the surface membrane of mouse thymocytes. These results show that B cells with reactivity towards different intracellular structures are present in the normal B cell repertoire whereas surface reactive B cells may be deleted or more profoundly suppressed or anergic and unable to form viable Ig producing hybridomas.