Effects of antimicrobial peptides on serum biochemical parameters, antioxidant activity and non-specific immune responses in Epinephelus coioides
Effects of antimicrobial peptides on serum biochemical parameters, antioxidant activity and non-specific immune responses in Epinephelus coioides
复制标题
抗菌肽对斜带石斑鱼血清生化参数、抗氧化活性和非特异性免疫反应的影响
DOI：
10.1016/j.fsi.2018.12.056
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发表时间：
2019-03-01
影响因子：
4.7
通讯作者：
Sun, Hong-Yan
中科院分区：
文献类型：
--
作者：
Su, Yu-Ling;Chen, Guo;Sun, Hong-Yan
抗菌肽（Antimicrobial Peptides，AMP）是一类具有广谱抗菌活性的小分子蛋白质，是一种有效的抗病原体（细菌、真菌和病毒）的药物。研究了枯草芽孢杆菌抗菌肽对斜带石斑鱼生长的影响。E.用含AMP（0、100、200、400或800 mg/kg）的饲料喂养Coioides 4周。结果表明：在实验组中，E.与对照组相比，大肠杆菌的总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和溶菌酶（LZM）水平均显著降低。此外，还观察了不同剂量AMP饲料对大肠杆菌组织中肿瘤坏死因子α（TNF-α）、白细胞介素1 β（IL-1 β）和热休克蛋白90（Hsp 90）mRNA表达的影响。当测量coioides时，所检查的组织中的三个基因显著上调。结果表明，添加抗菌肽能提高大肠杆菌的抗氧化能力和天然免疫能力。表明抗菌肽有可能作为抗生素的潜在替代药物。coioides。
Antimicrobial peptides (AMPs) are small proteins showing broad-spectrum antimicrobial activity that have been known to be powerful agents against a variety of pathogens (bacteria, fungi and viruses). In this study, the effects of AMPs from Bacillus subtilis on Epinephelus coioides were examined. E. coioides were fed with diets containing AMPs (0, 100, 200, 400 or 800 mg/kg) for four weeks. Results showed that the levels of total protein (TP), albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and blood glucose (GLU) and lipopolysaccharide (LPS) in the serum of E. coioides changed than those of the control group; compared to the control group, the levels of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and lysozyme (LZM) levels in E. coioides fed with different dosages AMP diets were also different; in addition, the mRNA expression of tumor necrosis factor alpha (TNF-alpha), interleukin-1-beta (IL-1 beta), and heat shock protein 90 (Hsp90) in the tissues of E. coioides were measured, the three genes in the tissues examined were significantly upregulated. The results demonstrated that diets containing AMPs can enhance the antioxidant capacity and innate immune ability of E. coioides, indicating that AMPs might be a potential alternative to antibiotics in E. coioides.