EVALUATION OF A BLOCKING ELISA USING MONOCLONAL-ANTIBODIES FOR THE DETECTION OF PORCINE EPIDEMIC DIARRHEA VIRUS AND ITS ANTIBODIES
EVALUATION OF A BLOCKING ELISA USING MONOCLONAL-ANTIBODIES FOR THE DETECTION OF PORCINE EPIDEMIC DIARRHEA VIRUS AND ITS ANTIBODIES
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DOI：
10.1177/104063879500700109
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发表时间：
1995-01-01
影响因子：
1.5
通讯作者：
CARMENES, P
中科院分区：
文献类型：
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作者：
CARVAJAL, A;LANZA, I;CARMENES, P
采用单克隆抗体（MAb）作为捕获和检测试剂的阻断酶联免疫吸附试验（ELISAs）和间接荧光试验（IFT）检测猪流行性腹泻病毒（PEDV）抗原或抗体。在用于病毒抗原检测的ELISA中，通过将粪便样品在一式两份的威尔斯孔中与PEDV特异性阳性或阴性血清孵育来完成封闭步骤，而在用于抗体检测的ELISA中，通过将血清样品与肠源性病毒悬浮液孵育来完成封闭步骤。所开发的所有方法都用于监测仔猪中PEDV CV-777株的实验感染和猪育肥单位中的自然FED爆发。抗原检测ELISA能够比先前描述的方法检测PEDV脱落更长的时间。用于抗体检测的阻断ELISA能够在PEDV感染后比IFT更快地检测血清抗体应答。
Two blocking enzyme-linked immunosorbent assays (ELISAs) involving the use of monoclonal antibodies (MAb) as capture and detecting agents and an indirect fluorescence test (IFT) were used for the detection of porcine epidemic diarrhea virus (PEDV) antigen or its antibodies. In the ELISA for viral antigen detection, the blocking step is accomplished by incubation of fecal samples, in duplicate wells, with PEDV-specific positive or negative serum, whereas in the ELISA for antibody detection the blocking step is accomplished by incubation of serum samples with a gut-origin virus suspension. All the methods developed were used to monitor an experimental infection in piglets with the CV-777 strain of PEDV and a natural FED outbreak in a swine fattening unit. The antigen-detection ELISA was able to detect PEDV shedding for a longer time than have previously described methods. The blocking ELISA for antibody detection was able to detect the serum antibody response sooner after PEDV infection than did IFT.