Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2
Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2
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DOI：
10.1126/science.276.5318.1571
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发表时间：
1997-06-06
期刊：
影响因子：
56.9
通讯作者：
Bokoch, GM
中科院分区：
文献类型：
--
作者：
Rudel, T;Bokoch, GM
Jurkat T细胞凋亡诱导caspase介导的p21激活激酶2（PAK2）的蛋白水解裂解。氨基末端调节结构域和羧基末端催化结构域之间发生裂解，产生组成型活性PAK2片段。稳定的Jurkat细胞株，表达显性负PAK突变体的抗Fas诱导的凋亡小体的形成，但有一个增强的磷脂酰丝氨酸在细胞表面的外部化。因此，PAK2的蛋白水解激活代表了PAK调节的鸟苷三磷酸酶独立机制，其允许PAK2调节在凋亡细胞中观察到的形态学变化。
Apoptosis of Jurkat T cells induced the caspase-mediated proteolytic cleavage of p21-activated kinase 2 (PAK2). Cleavage occurred between the amino-terminal regulatory domain and the carboxyl-terminal catalytic domain, which generated a constitutively active PAK2 fragment. Stable Jurkat cell lines that expressed a dominant-negative PAK mutant were resistant to the Fas-induced formation of apoptotic bodies, but had an enhanced externalization of phosphatidlyserine at the cell surface. Thus, proteolytic activation of PAK2 represents a guanosine triphosphatase-independent mechanism of PAK regulation that allows PAK2 to regulate morphological changes that are seen in apoptotic cells.