Trichokonins from Trichoderma pseudokoningii SMF2 induce resistance against Gram-negative Pectobacterium carotovorum subsp carotovorum in Chinese cabbage
Trichokonins from Trichoderma pseudokoningii SMF2 induce resistance against Gram-negative Pectobacterium carotovorum subsp carotovorum in Chinese cabbage
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来自假康宁木霉 SMF2 的木霉素诱导大白菜对革兰氏阴性胡萝卜果杆菌胡萝卜亚种的抗性
DOI：
10.1111/1574-6968.12427
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发表时间：
2014-05-01
影响因子：
2.1
通讯作者：
Song, Xiao-Yan
中科院分区：
文献类型：
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作者：
Li, Hai-Yun;Luo, Yan;Song, Xiao-Yan
木霉产生的抑菌肽在防治真菌、病毒和革兰氏阳性菌引起的植物病害中发挥着重要作用。本研究以拟康氏木霉（Trichoderma pseudokoningii SMF 2）的抗菌肽Trichokonins对由革兰氏阴性细菌胡萝卜软腐病菌（Pectobacterium carotovorum subsp.）胡萝卜素降解的研究，并分析了其作用机制。0.3mgL ~（-1）浓度的毛菌素处理提高了大白菜对Pcc侵染的抗性。但在体外培养条件下，即使在高浓度（500 mgL ~（-1））下，毛扣素对Pcc的生长也几乎没有抑制作用。因此，Trichokonins对Pcc的直接作用可能不是Trichokonins防治大白菜软腐病的主要原因。天花粉蛋白处理导致活性氧过氧化氢和超氧阴离子自由基的产生明显增加，过氧化氢酶、多酚氧化酶和过氧化物酶活性显著增强，水杨酸响应的病程相关蛋白基因酸性PR-1a的表达上调。这些结果表明，木霉菌素通过激活水杨酸信号通路诱导大白菜对Pcc侵染产生抗性，表明木霉菌和肽霉菌素在防治植物病害中具有潜在的应用价值。
Peptaibols, mainly produced by Trichoderma, play a pivotal role in controlling plant disease caused by fungi, virus, and Gram-positive bacteria. In the current study, we evaluated the control effect of Trichokonins, antimicrobial peptaibols from Trichoderma pseudokoningii SMF2, on soft rot disease of Chinese cabbage caused by a Gram-negative bacterium Pectobacterium carotovorum subsp. carotovorum and analyzed the mechanism involved. Trichokonins treatment (0.3mgL(-1)) enhanced the resistance of Chinese cabbage against Pcc infection. However, Trichokonins could hardly inhibit the growth of Pcc in vitro, even at high concentration (500mgL(-1)). Therefore, the direct effect of Trichokonins on Pcc may not the main reason why Trichokonins could control soft rot of Chinese cabbage. Trichokonin treatment led to an obvious increase in the production of reactive oxygen species hydrogen peroxide and superoxide radical, a significant enhance of the activities of pathogenesis-related enzymes catalase, polyphenoloxidase and peroxidase, and upregulation of the expression of salicylic acid - responsive pathogenesis-related protein gene acidic PR-1a in Chinese cabbage. These results indicate that Trichokonins induce resistance in Chinese cabbage against Pcc infection through the activation of salicylic acid signaling pathway, which imply the potential of Trichoderma and peptaibols in controlling plant disease caused by Gram-negative bacteria.