Parental centrioles are dispensable for deuterosome formation and function during basal body amplification
Parental centrioles are dispensable for deuterosome formation and function during basal body amplification
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亲代中心粒对于基底体扩增过程中后体的形成和功能是可有可无的
DOI：
10.15252/embr.201846735
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发表时间：
--
期刊：
影响因子：
7.7
通讯作者：
Zhu Xueliang
中科院分区：
文献类型：
--
作者：
Zhao Huijie;Chen Qingxia;Fang Chuyu;Huang Qiongping;Zhou Jun;Yan Xiumin;Zhu Xueliang
哺乳动物上皮细胞利用一对亲代中心粒和大量的后体作为平台，在多发性髂骨发生过程中高效地生产基底体。然而，后体的形成和功能仍然存在争议。它们被认为是为了大量的从头中心粒生物发生而自发产生的，或者以依赖于女儿中心粒的方式出现，作为航天飞机带走聚集在中心粒上的前着丝粒。在这里，我们证明了两个亲本中心粒对于后体的形成是必不可少的。在小鼠气管上皮细胞和室管膜细胞(mTECs和MEPCs)中，细胞质中离散的后体最初是无前着丝点的。它们出现在细胞质中广泛分散的位置，然后扩大，伴随着它们形成原着丝粒的能力增强。更重要的是，在MEPCS中，后体仍然有效地形成，其子代中心粒甚至双亲中心粒通过shRNA介导的Plk4的耗竭或药物抑制而被消除，Plk4是周期细胞和多纤毛细胞中中心粒生物发生所必需的一种激酶。因此，在多纤毛细胞中，后体可以自主组装以介导从头中心粒的扩增。
Mammalian epithelial cells use a pair of parental centrioles and numerous deuterosomes as platforms for efficient basal body production during multiciliogenesis. How deuterosomes form and function, however, remain controversial. They are proposed to arise either spontaneously for massive de novo centriole biogenesis or in a daughter centriole‐dependent manner as shuttles to carry away procentrioles assembled at the centriole. Here, we show that both parental centrioles are dispensable for deuterosome formation. In both mouse tracheal epithelial and ependymal cells (mTECs and mEPCs), discrete deuterosomes in the cytoplasm are initially procentriole‐free. They emerge at widely dispersed positions in the cytoplasm and then enlarge, concomitant with their increased ability to form procentrioles. More importantly, deuterosomes still form efficiently in mEPCs whose daughter centriole or even both parental centrioles are eliminated through shRNA‐mediated depletion or drug inhibition of Plk4, a kinase essential to centriole biogenesis in both cycling cells and multiciliated cells. Therefore, deuterosomes can be assembled autonomously to mediate de novo centriole amplification in multiciliated cells.