Phylogenetic analysis of classical swine fever virus in Taiwan
Phylogenetic analysis of classical swine fever virus in Taiwan
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DOI：
10.1007/s00705-004-0485-6
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发表时间：
2005-06-01
影响因子：
2.7
通讯作者：
Lai, SS
中科院分区：
文献类型：
--
作者：
Pan, CH;Jong, MH;Lai, SS
通过 RT-PCR 扩增了猪瘟病毒 (CSFV) 的两个包膜糖蛋白（E-rns 和 E2）区域，并直接对 1989 年至 2003 年间在台湾收集的 158 个样本进行测序。这两个区域的系统发育分析揭示了相似的树拓扑，并且 E-rns 区域比 E2 区域提供了更好的区分度。 158 个分离株中的 115 个分离株聚集在亚组 2.1（进一步分类为 2.1a 和 2.1b）和 2.2 内，被认为可能是引入菌株，而其余 43 个分离株聚集在亚组 3.4 内，被认为是地方性菌株。 2.1a 亚组病毒于 1994 年首次被发现，并从 1995 年起占据主导地位。然而，亚型 3.4 病毒在早年很流行，1996 年之后就没有被分离出来。我们观察到基因型从亚型 3.4 到 2.1a 发生了巨大的转变。亚组2.1a分离株与帕德博恩和老挝分离株密切相关，而2.1b分离株与中国广西分离株有密切关系。基于完整包膜糖蛋白基因（E-rns-E2）的27个CSFV序列的系统发育树比基于完整开放阅读框的系统发育树显示出更好的分辨率。
Two envelope glycoprotein (E-rns and E2) regions of the classical swine fever virus (CSFV) were amplified by RT-PCR and sequenced directly from 158 specimens collected between 1989 and 2003 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and the E-rns region provided better discrimination than the E2 region. One hundred and fifteen isolates out of the 158 isolates were clustered within subgroup 2.1 (further classified as 2.1a and 2.1b) and 2.2, which were considered to be likely of the introduced strains, whereas the remaining 43 isolates were clustered within subgroup 3.4 and were considered to be of the endemic strains. The subgroup 2.1a viruses were first detected in 1994 and predominated from 1995 onwards. However, subgroup 3.4 viruses were prevalent in the early years, not being isolated after 1996. We have observed a dramatic switch in genotype from subgroup 3.4 to 2.1a. The subgroup 2.1a isolates are closely related to the Paderborn and Lao isolates, whereas 2.1b isolates have a close relationship to the Chinese Guangxi isolates. The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (E-rns-E2) displayed better resolution than that based on the complete open reading frame.