A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation.
A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation.
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DOI：
10.1186/1758-907x-2-4
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发表时间：
2011-06-07
期刊：
影响因子：
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通讯作者：
Zamore PD
中科院分区：
文献类型：
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作者：
Seitz H;Tushir JS;Zamore PD
微小RNA（miRNA）序列多样，并且具有单一已知的序列偏好：它们倾向于以尿苷（U）开始。我们对果蝇、蠕虫和小鼠miRNA序列数据的分析表明，5′-U在miRNA产生后被识别。两条链中只有一条可以从单个miRNA/miRNA* 分子组装成Argonaute蛋白：在苍蝇胚胎裂解物中，5′-U促进miRNA加载，同时降低miRNA* 的加载。我们认为，5′-U的识别增强Argonaute加载的机制与其在潜在miRNA的5′-末端削弱碱基配对的贡献不同，并且最近在拟南芥和人类中提出，它通过排除携带其他5′-nt的错误加工分子来提高miRNA的精度。
MicroRNA (miRNA) are diverse in sequence and have a single known sequence bias: they tend to start with uridine (U). Our analyses of fly, worm and mouse miRNA sequence data reveal that the 5′-U is recognized after miRNA production. Only one of the two strands can be assembled into Argonaute protein from a single miRNA/miRNA* molecule: in fly embryo lysate, a 5′-U promotes miRNA loading while decreasing the loading of the miRNA*. We suggest that recognition of the 5′-U enhances Argonaute loading by a mechanism distinct from its contribution to weakening base pairing at the 5′-end of the prospective miRNA and, as recently proposed in Arabidopsis and in humans, that it improves miRNA precision by excluding incorrectly processed molecules bearing other 5′-nt.