Preliminary observations of genetic susceptibility of elk (Cervus elaphus nelsoni) to chronic wasting disease by experimental oral inoculation
Preliminary observations of genetic susceptibility of elk (Cervus elaphus nelsoni) to chronic wasting disease by experimental oral inoculation
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DOI：
10.1177/104063870601800118
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发表时间：
2006-01-01
影响因子：
1.5
通讯作者：
O'Rourke, KI
中科院分区：
文献类型：
--
作者：
Hamir, AN;Gidlewski, T;O'Rourke, KI
为了比较具有PRNP基因（编码正常细胞朊蛋白）不同等位基因的麋鹿（Cervus elaphus nelsoni）对慢性消耗性疾病（CWD）的遗传易感性，对8头8月龄3种基因型（2 132 MM、2 132 LM和4 132 LL）的麋鹿犊牛口服感染CWD的脑组织。在接种后（PI）第23个月期间，两只132 MM麋鹿均食欲不振，出现体重减轻和中枢神经系统（CNS）功能障碍的临床体征，并被处以安乐死。另外两只麋鹿（均为132 LM）出现了相似的疾病临床体征，并在PI第40个月期间被处以安乐死。所有4个受影响的麋鹿海绵状脑病（SE）的显微镜下病变，和PrPres，疾病相关形式的朊病毒蛋白，检测在其中枢神经系统和淋巴组织中使用免疫组化（IHC）和蛋白质印迹（WB）技术。这些发现表明，在密码子132处具有MM和LM的麋鹿对经口接种的CWD敏感。所有4只LL麋鹿在PI第4年均存活，且临床正常，这表明132 LL麋鹿对CWD感染物质的口腔感染的易感性可能降低，或者可能潜伏时间延长。
To compare the genetic susceptibility of elk (Cervus elaphus nelsoni) with various alleles of the PRNP gene, which encodes the normal cellular prion protein, to chronic wasting disease (CWD), eight 8-month-old elk calves of 3 genotypes (2 132MM, 2 132LM, and 4 132LL) were orally dosed with CWD-infected brain material from elk. During postinoculation (PI) month 23, both 132MM elk had lost appetite, developed clinical signs of weight loss and central nervous system (CNS) dysfunction, and were euthanized. Two other elk (both 132LM) developed similar clinical signs of disease and were euthanized during PI month 40. All 4 affected elk had microscopic lesions of spongiform encephalopathy (SE), and PrPres, the disease-associated form of the prion protein, was detected in their CNS and lymphoid tissues by use of immunohistochemical (IHC) and Western blot (WB) techniques. These findings indicate that elk with MM and LM at codon 132 are susceptible to orally inoculated CWD. All 4 LL elk are alive at PI year 4 and are clinically normal, which suggests that 132LL elk may have reduced susceptibility to oral infection with CWD-infected material or may have prolonged incubation time.