MATRIX DAMAGE AND CHONDROCYTE VIABILITY FOLLOWING A SINGLE IMPACT LOAD ON ARTICULAR-CARTILAGE
MATRIX DAMAGE AND CHONDROCYTE VIABILITY FOLLOWING A SINGLE IMPACT LOAD ON ARTICULAR-CARTILAGE
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DOI：
10.1006/abbi.1995.1439
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发表时间：
1995-09-10
影响因子：
3.9
通讯作者：
ASPDEN, RM
中科院分区：
文献类型：
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作者：
JEFFREY, JE;GREGORY, DW;ASPDEN, RM
在体外对关节软骨的全深度圆形样品施加冲击载荷，研究冲击能量和速度对基质完整性和软骨细胞活力的影响。在一次严重的撞击后，计算出软骨表面的能量密度，这可能是一个人从1米高的墙上跳下来所预期的，组织被严重破坏，它变成椭圆形，裂缝，扁平。在相似的坠落质量和高度下，仍附着在下层骨上的肱骨样本显示出较小的损伤。软骨细胞的活力被认为是线性下降，增加冲击能量。在撞击后培养长达15天的软骨活检组织在前3天内获得了质量，随后没有丢失。质量的增加随着冲击的严重程度而增加，这是由于组织的水合作用增加。扫描电子显微镜和光学显微镜显示裂缝穿透组织，但从未发现穿过整个深度。它们通常与表面的平面成大约45度取向，并且在到达过渡区时给出平行于表面偏转的外观。这就产生了一种“分层"效应，表层与深层分离。(C)出版社：Academic Press
An impact load was applied to full-depth circular samples of articular cartilage in vitro and the effects of impact energy and velocity on matrix integrity and chondrocyte viability were studied. Following a severe impact, calculated to correspond to the energy density over the cartilage surface that might be expected in a manjumping off a 1-m-high wall, the tissue was grossly disrupted, It became elliptical, fissured, and flattened. Cartilage samples remaining attached to the underlying bone showed less damage at similar drop masses and heights. Chondrocyte viability was found to decrease linearly with increasing impact energy. Cartilage biopsies maintained in culture for up to 15 days following impact gained mass over the first 3 days which they did not subsequently lose. The gain in mass increased with the severity of impact and was due to an increased hydration of the tissue. Scanning electron microscopy and light microscopy showed fissures penetrating the tissue but which were never found to pass through the full depth. They were commonly oriented at about 45 degrees to the plane of the surface and gave the appearance of being deflected parallel to the surface on reaching the transition zone. This produced a ''delaminating'' effect where the surface zone was separating from the deep zone. (C) 1995 Academic Press, Inc.