The role of reactive oxygen species and apoptosis in the pathogenesis of varicocele in a rat model and efficiency of vitamin E treatment.
The role of reactive oxygen species and apoptosis in the pathogenesis of varicocele in a rat model and efficiency of vitamin E treatment.
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DOI：
10.1111/j.1365-2605.2004.00476.x
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发表时间：
2004-08
影响因子：
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通讯作者：
K. Çam;F. Şimşek;Meral Yūksel;L. Turkeri;G. Haklar;S. Yalcin;A. Akdaş
中科院分区：
文献类型：
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作者：
K. Çam;F. Şimşek;Meral Yūksel;L. Turkeri;G. Haklar;S. Yalcin;A. Akdaş
本研究采用精索静脉曲张动物模型，探讨活性氧（ROS）和细胞凋亡在不育症发病机制中的作用。还检查了维生素E的保护作用。第一组为假手术组，后两组采用部分结扎左肾静脉的方法建立实验性精索静脉曲张模型。第三组给予维生素E。用化学发光法（CL）测定ROS的产生。原位末端标记技术用于研究细胞凋亡。组织维生素E水平测定高效液相色谱法。各组间鲁米诺增强CL水平差异无统计学意义。然而，在假手术组和精索静脉曲张组的左睾丸中光泽精探针的CL水平之间的差异具有统计学显著性（p = 0.0007）。同样，接受维生素E的第三组的结果与精索静脉曲张组有显著差异（p = 0.0025）。假手术组和精索静脉曲张组之间的凋亡指数差异也具有统计学意义（p = 0.0038）。虽然维生素E组的凋亡指数值低于精索静脉曲张组，但差异无统计学意义。本研究提示精索静脉曲张可诱导睾丸内ROS产生和细胞凋亡。维生素E具有保护作用。实验性精索静脉曲张的细胞凋亡率增加表明分子改变，这可能涉及ROS过度产生作为触发机制。因此，这项研究表明精索静脉曲张和不孕症之间的联系在分子水平上通过刺激活性氧和细胞凋亡。
We investigated role of reactive oxygen species (ROS) and apoptosis in the pathogenesis of infertility in experimental model of varicocele. The protective effect of vitamin E was also examined. Three groups of rats were constructed as the first group had sham operation, experimental varicoceles were established by partial ligation of the left renal vein in later two groups. Third group had received vitamin E. Production of ROS was determined by chemiluminescence assay (CL). The in situ end labelling technique was utilized to investigate apoptosis. Tissue vitamin E levels were measured by high performance liquid chromatography. The differences between luminol enhanced CL levels of groups were not statistically significant. However, the difference between CL levels of lucigenin probe in left testicles of sham and varicocele groups were statistically significant ( p = 0.0007). Similarly, the results of the third group receiving vitamin E significantly differed from the varicocele group ( p = 0.0025). The difference of apoptotic index was also statistically significant between sham and varicocele groups ( p = 0.0038). Although the values of apoptotic index detected in the vitamin E group were lower compared with the varicocele group, the difference was not significant. This study proposes that ROS production and apoptosis in the testicles were induced with experimental varicocele. Vitamin E had a protective role. An increased rate of apoptosis with experimental varicocele suggests a molecular alteration, which may involve ROS overproduction as the triggering mechanism. Consequently, this study indicates an association between varicocele and infertility at molecular level through stimulation of ROS and apoptosis.