Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control
Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control
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DOI：
10.1371/journal.pone.0228596
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发表时间：
2020-02-06
期刊：
影响因子：
3.7
通讯作者：
Melchers, Willem J. G.
中科院分区：
文献类型：
--
作者：
Machiels, Julian D.;Cremers, Amelieke J. H.;Melchers, Willem J. G.
胃肠道感染的常规PCR检测通常是基于临床医生特别要求的病原体相关面板，可能是错误的和耗时的。BioFire FilmArray胃肠道（GI）检测试剂盒在随机存取系统上将22种病原体合并到一个基于细菌的检测中，从而将周转时间缩短至不到2小时。我们描述了实施BioFire FilmArray对胃肠炎患者在我们的hospital.MethodsPatients参加荷兰三级保健中心（Radboud大学医学中心），从其中获得粪便样本的临床影响，有资格列入。临床医生根据临床病史和症状选择一种或多种不同的常规PCR检测板（细菌检测板、病毒检测板、梭菌检测板和三种寄生虫检测板）。使用FilmArray对所有样本进行平行检测。我们回顾性地收集了有关感染控制和患者管理的患者数据，以评估实施FilmArray.ResultsIn共182例患者的潜在影响。常规PCR在52例（28.6%）患者中检出了一种或多种病原体，而使用FilmArray时为72例（39.6%）。周转时间（包括运输）从常规PCR的中位53小时缩短至FilmArray的16小时。26例患者可以从隔离早29小时，3.6抗生素天可以被保存，并在5例患者额外的成像测试（包括结肠镜检查）可能已经prevent.ConclusionThe理论实施的BioFire FilmArray GI面板在临床疑似胃肠炎的患者导致了显着更好的患者管理。
ObjectivesConventional routine PCR testing for gastrointestinal infections is generally based on pathogen related panels specifically requested by clinicians and can be erroneous and time consuming. The BioFire FilmArray gastrointestinal (GI) panel combines 22 pathogens into a single cartridge-based test on a random-access system, thereby reducing the turnaround time to less than 2 hours. We described the clinical impact of implementing the BioFire FilmArray on patients with gastroenteritis in our hospital.MethodsPatients attending a Dutch tertiary care center (Radboud University Medical Center), from whom stool samples were obtained, were eligible for inclusion. The clinicians selected one or a combination of different routinely performed PCR panels (bacterial panel, viral panel, clostridium testing, and three parasitic panels) based on clinical history and symptoms. All samples were in parallel tested with the FilmArray. We retrospectively collected patient data regarding infection control and patient management to assess the potential impact of implementing the FilmArray.ResultsIn total 182 patients were included. Routine PCR detected one or more pathogens in 52 (28.6%) patients compared to 72 (39.6%) using the FilmArray. Turnaround time (including transport) decreased from median 53 hours for the routine PCR to 16 hours for the FilmArray. Twenty-six patients could have been removed from isolation 29 hours sooner, 3.6 antibiotic days could have been saved and in five patients additional imaging testing (including colonoscopies) could have been prevented.ConclusionThe theoretical implementation of the BioFire FilmArray GI panel in patients with clinical suspicion of gastroenteritis resulted in a significant better patient management.