INTERACTION + ASSOCIATION OF BASES + NUCLEOSIDES IN AQUEOUS SOLUTIONS .3. NUCLEAR MAGNETIC RESONANCE STUDY OF SELF-ASSOCIATION OF PURINE + 6-METHYLPURINE
INTERACTION + ASSOCIATION OF BASES + NUCLEOSIDES IN AQUEOUS SOLUTIONS .3. NUCLEAR MAGNETIC RESONANCE STUDY OF SELF-ASSOCIATION OF PURINE + 6-METHYLPURINE
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DOI：
10.1021/ja01073a055
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发表时间：
1964-01-01
影响因子：
15
通讯作者：
HELMKAMP, GK
中科院分区：
文献类型：
--
作者：
CHAN, SI;SCHWEIZER, MP;HELMKAMP, GK
碱基和核苷在水溶液中的结合最近通过渗透作用的研究得到了证实。为了进一步阐明这种相互作用的性质，现已详细研究了嘌呤和6-甲基嘌呤的质子磁共振谱随浓度的变化。观察到了明显的集中效应。质子磁共振谱的这种浓度依赖关系被发现既与温度有关，也与溶剂有关。这些数据，再加上有关嘌呤盐酸盐的其他结果，证实了渗透研究的解释，并进一步有力地表明，缔合模式是环以部分重叠的方式垂直堆积的模式。模型计算表明，核磁共振结果与渗透压结果定量一致，表明该络合物的平均环间距为3~4A。
The association of bases and nucleosides in aqueous solutions has recently been established by osmotic studies. To further elucidate the nature of this association, the proton magnetic resonance spectra of purine and 6-methylpurine have now been studied in detail as a function of concentration. A pronounced concentration effect has been observed. This concentration dependence of the proton magnetic resonance spectra was found to be both temperature and solvent dependent. These data, together with additional results on purine hydrochlo-ride, substantiate the interpretations of the osmotic studies and further strongly suggest that the mode of association is that of vertical stacking of rings in a partial overlapping fashion. Model calculations show that the nmr results are in quantitative agreement with the osmotic results and indicate that the average distance between rings in the complex is 3 to 4 A.