Intracellular chloride ion concentration in differentiating neuronal cell and its role in growing neurite.
Intracellular chloride ion concentration in differentiating neuronal cell and its role in growing neurite.
复制标题
分化神经细胞中的细胞内氯离子浓度及其在神经突生长中的作用。
DOI：
10.1016/j.bbrc.2016.09.075
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Marunaka Y
中科院分区：
文献类型：
--
作者：
Nakajima K;Marunaka Y
氯离子(Cl-−)是人体内含量最丰富的阴离子之一。越来越多的证据表明，Cl-−在多种细胞功能中发挥着重要作用。我们已经报道了电子中性型阳离子转运体，如Na+-K+-2Cl型−共转运体1(NKCC1)和K+-Cl型−共转运体1(KCC1)，参与了神经元分化过程中轴突的生长。在本研究中，我们研究了细胞内氯−浓度([Cl−]i)与生长轴突的长度之间是否存在相关性。我们用卤化物敏感的荧光dyeN-(ethoxycarbonylmethyl)-6-methoxyquinolinium溴化物测定了细胞体和生长中的轴突顶端的[Cl−]i，发现在单个细胞中，生长的轴突顶端的[Cl−]i高于细胞体。重要的是，生长轴突的长度与轴突尖端的[Cl−]i呈显著正相关。NKCC1抑制剂丁酰亚胺可显著抑制突起生长，降低突起尖端[Cl−]i。本研究和我们以前的研究结果有力地表明，高[Cl−]i的轴突尖端区域对于高效的轴突生长是至关重要的。
Chloride ion (Cl−) is one of the most abundant anions in our body. Increasing evidence suggests that Cl−plays fundamental roles in various cellular functions. We have previously reported that electroneutral cation-chloride cotransporters, such as Na+-K+-2Cl−cotransporter 1 (NKCC1) and K+-Cl−cotransporter 1 (KCC1), are involved in neurite outgrowth during neuronal differentiation. In the present study, we studied if there is correlation between intracellular Cl−concentrations ([Cl−]i) and the length of growing neurites. We measured [Cl−]iin the cell body and growing neurite tips using halide-sensitive fluorescent dyeN-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE), revealing that [Cl−]iin the tip of growing neurite was higher than that in cell body in a single cell. Importantly, there was a significant positive correlation between the length of growing neurite and [Cl−]iin neurite tip. Bumtanide (BMT), an inhibitor of NKCC1, significantly inhibited neurite outgrowth and decreased [Cl−]iin neurite tip. The results obtained in the present study and our previous studies together strongly suggest that high [Cl−]iin neurite tip region is crucial for efficient neurite outgrowth.