INTERFERON-GAMMA DIRECTLY AFFECTS BARRIER FUNCTION OF CULTURED INTESTINAL EPITHELIAL MONOLAYERS
INTERFERON-GAMMA DIRECTLY AFFECTS BARRIER FUNCTION OF CULTURED INTESTINAL EPITHELIAL MONOLAYERS
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DOI：
10.1172/jci113938
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发表时间：
1989-02-01
影响因子：
15.9
通讯作者：
STAFFORD, J
中科院分区：
文献类型：
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作者：
MADARA, JL;STAFFORD, J
尽管上皮细胞常常与淋巴细胞紧密接触，并且可能具有各种细胞因子的受体，但细胞因子是否直接影响上皮细胞的功能尚不清楚。我们研究了细胞因子干扰素（IFN）对培养的T84人肠上皮细胞系单层的屏障功能的影响。γ干扰素在显示其其他生物学效应所需的浓度和暴露条件下，直接影响这种单层细胞。γ干扰素使单层细胞的电阻显著降低。从形态学和乳酸脱氢酶（LDH）测定判断，这种效应不是由于细胞毒性。溶质通量和双钠 - 甘露醇通量分析表明，电阻降低是由于γ干扰素对紧密连接通透性的影响。白细胞介素1、白细胞介素2或肿瘤坏死因子不能复制γ干扰素对单层屏障功能的影响。我们推测，淋巴细胞激活的此类产物可能在疾病状态下影响肠道以及也许其他上皮细胞的屏障功能。
Although epithelia, which often are in intimate contact with lymphoid cells, may bear receptors for various cytokines, it is unclear whether cytokines directly effect epithelial function. We examine the effects of the cytokine interferon (IFN) on barrier function of cultured monolayers of the T84 human intestinal epithelial cell line. .gamma.IFN, in concentrations and exposures required to show its other biological effects, directly affects such monolayers. Monolayer resistance is substantially diminished by .gamma.IFN. Such effects were not due to cytotoxicity as judged morphologically and by LDH assays. Solute fluxes and dual Na+-mannitol flux analysis indicate that the resistant decrease is due to an effect of .gamma.IFN on tight junction permeability. The effects of .gamma.IFN on monolayer barrier function were not duplicated by the cytokines interleukin 1, interleukin 2, or tumor necrosis factor. We speculate that such products of activation of lymphoid cells might influence barrier function of intestinal, and perhaps other epithelia in disease states.