Bactericidal efficacy of atmospheric pressure non-thermal plasma (APNTP) against the ESKAPE pathogens
Bactericidal efficacy of atmospheric pressure non-thermal plasma (APNTP) against the ESKAPE pathogens
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DOI：
10.1016/j.ijantimicag.2015.02.026
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发表时间：
2015-07-01
影响因子：
10.8
通讯作者：
Gilmore, Brendan F.
中科院分区：
文献类型：
--
作者：
Flynn, Padrig B.;Higginbotham, Sarah;Gilmore, Brendan F.
临床环境中多药耐药病原体的出现给全球医院带来了日益严重的问题。“ESKAPE”病原体（屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属）在大多数医院感染中作为一组致病微生物而被强调，由于广泛的抗微生物耐药性，呈现出严重的健康风险。新抗生素的停滞管道需要替代方法来控制和治疗医院感染。大气压非热等离子体（APNTP）作为临床环境中的替代感染控制方法正吸引越来越多的兴趣。这项研究提出了一个全面的杀菌评估的内部设计的APNTP射流对生物膜和寄生菌的ESKAPE病原体。使用标准平板计数和XTT代谢试验评价APNTP的抗菌作用，两种方法均显示出相当的根除时间。APNTP对所有ESKAPE病原菌均表现出快速的抗菌活性，并在360 s内有效和完全根除ESKAPE病原菌，但A.其中观察到生物膜活力的> 4log减少。这证明了其作为针对这些病原体的杀菌治疗的有效性，并进一步突出了其在临床环境中用于控制高度抗微生物剂耐药性病原体的潜在应用。(C)2015年Elsevier B.V.和国际化疗学会。All rights reserved.
The emergence of multidrug-resistant pathogens within the clinical environment is presenting a mounting problem in hospitals worldwide. The 'ESKAPE' pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae,Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) have been highlighted as a group of causative organisms in a majority of nosocomial infections, presenting a serious health risk due to widespread antimicrobial resistance. The stagnating pipeline of new antibiotics requires alternative approaches to the control and treatment of nosocomial infections. Atmospheric pressure non-thermal plasma (APNTP) is attracting growing interest as an alternative infection control approach within the clinical setting. This study presents a comprehensive bactericidal assessment of an in-house-designed APNTP jet both against biofilms and planktonic bacteria of the ESKAPE pathogens. Standard plate counts and the XTT metabolic assay were used to evaluate the antibacterial effect of APNTP, with both methods demonstrating comparable eradication times. APNTP exhibited rapid antimicrobial activity against all of the ESKAPE pathogens in the planktonic mode of growth and provided efficient and complete eradication of ESKAPE pathogens in the biofilm mode of growth within 360 s, with the exception of A. baumannii where a >4 log reduction in biofilm viability was observed. This demonstrates its effectiveness as a bactericidal treatment against these pathogens and further highlights its potential application in the clinical environment for the control of highly antimicrobial-resistant pathogens. (C) 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.