CLONING OF THE ZEBRAFISH KROX-20 GENE (KRX-20) AND ITS EXPRESSION DURING HINDBRAIN DEVELOPMENT
CLONING OF THE ZEBRAFISH KROX-20 GENE (KRX-20) AND ITS EXPRESSION DURING HINDBRAIN DEVELOPMENT
复制标题
DOI：
10.1093/nar/21.5.1087
复制
发表时间：
1993-03-11
影响因子：
14.9
通讯作者：
JOWETT, T
中科院分区：
文献类型：
--
作者：
OXTOBY, E;JOWETT, T
为了开始研究控制后脑早期发育的基因的功能，我们用含有保守锌指区的鼠krox-20基因探针筛选了胚胎斑马鱼cDNA文库。我们已经分离出两个重叠的cDNA，zf 187和zf 201，它们是小鼠krox-20基因的同源物。最长的cDNA（zf 201）的N-末端含有两个与鼠krox-20相同的酸性区域。这表明这些蛋白质的功能组织可能是保守的。北方印迹分析鉴定了2.0 kb的单一转录本。全血原位杂交建立了表达的斑马鱼基因（kirp-20）首先出现在100% epiboly作为一个单一的前域的前瞻性神经上皮，其次是第二个更后域后不久。该基因在菱体（r）r3和r5中的交替表达模式在受精后12小时是明显的，即在菱体的形态外观之前。在14小时左右，神经嵴从r5的背侧开始迁移，向尾侧迁移到r6，然后向腹侧迁移到咽弓。嵴迁移在16小时或之后不明显。从r3开始没有观察到神经嵴迁移。kappa-20的表达首先在r3约26小时下调，随后在r5约30小时下调。
To begin to examine the function of genes that control early development in the hindbrain, we have screened an embryonic zebrafish cDNA library with a murine krox-20 gene probe that contained the conserved zinc finger regions. We have isolated two overlapping cDNAs, zf187 and zf201 which are homologues of the murine krox-20 gene. The N-terminal of the longest cDNA (zf201) contains two acidic regions identical to those of the murine krox-20. This indicates that the functional organisation of these proteins is probably conserved. Northern Blot analysis identified a single transcript of 2.0 kb. Wholemount in situ hybridisation established that expression of the zebrafish gene (krx-20) first appears at 100% epiboly as a single anterior domain of the prospective neuroepithelium, followed very soon after by a second more posterior domain. The alternating pattern of expression of this gene in rhombomeres(r) r3 and r5 is apparent by 12 hr post-fertilisation, that is prior to the morphological appearance of the rhombomeres. Around 14 hr neural crest migration begins from the dorsal surface of r5, moving caudally into r6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16 hr. No neural crest migration was observed from r3. Expression of krx-20 is down regulated firstly in r3 around 26 hr and later in r5 around 30 hr.