HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions
HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions
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DOI：
10.1101/gr.147400
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发表时间：
2000-10-01
期刊：
影响因子：
7
通讯作者：
Hirano, T
中科院分区：
文献类型：
--
作者：
Neuwald, AF;Hirano, T
HEAT重复对应于串联排列的卷曲状结构，这些结构似乎是其他组件可以组装的灵活脚手架。使用敏感的序列分析技术，我们检测到在各种染色体相关的蛋白质，包括四个家庭的蛋白质与凝聚素和凝聚素，这是核复合物，含有染色体（SMC）蛋白的结构维持热重复。检测到的蛋白质包括非洲爪蟾13 S缩合素复合物的XCAP-D2和XCAP-G亚基、曲霉菌BimD和大孢囊壳菌Spo 76 p蛋白、芽殖酵母Scc 2 p蛋白和相关的果蝇转录激活因子Nipped-B。网格蛋白衔接子和COP-I外被体亚基在囊泡外被装配中起作用，并且先前注意到与凝聚素亚基具有弱的序列相似性，它们也含有HEAT重复。在TBP相关的TIP 120蛋白（一种全局转录增强子）和芽殖酵母Motlp蛋白（SWI 2/SNF 2家族成员）中也发现了HEAT重复序列。SWI 2/SNF 2蛋白，其中一些是解旋酶，在转录控制，DNA修复和染色体分离中发挥不同的作用，并形成染色质重塑复合物。HEAT重复序列也发现在disl-TOG家族和辅因子D家族微管相关蛋白，由于其在微管动力学中的作用，执行与有丝分裂进程和染色体分离相关的功能。因此，我们的分析预测了这些蛋白质的结构特征，并表明HEAT重复序列可能在染色体动力学中发挥重要作用。
HEAT repeats correspond to tandemly arranged curlicue-like structures that appear to serve as flexible scaffolding on which other components can assemble. Using sensitive sequence analysis techniques we detected HEAT repeals in various chromosome-associated proteins, including four Families of proteins associated with condensins and cohesins, which are nuclear complexes that contain structural maintenance of chromosome (SMC) proteins. Among the proteins detected were the XCAP-D2 and XCAP-G subunits of the Xenopus laevis 13S condensin complex, the Aspergillus BimD and Sordaria macrospora Spo76p proteins, the budding yeast Scc2p protein, and the related Drosophila transcriptional activator Nipped-B. Clathrin adaptor and COP-I coatomer subunits, which Function in vesicle coat assembly and were previously noted to share weak sequence similarity to condensin subunits, also contain HEAT repeats. HEAT repeats were also found in the TBP-associated TIP120 protein, a global enhancer of transcription, and in the budding yeast Motlp protein, which is a member of the SWI2/SNF2 family. SWI2/SNF2 proteins, some of which are helicases, perform diverse roles in transcription control, DNA repair, and chromosome segregation and form chromatin-remodeling complexes. HEAT repeats also were found in disl-TOG family and cofactor D family microtubule-associated proteins, which, owing to their roles in microtubule dynamics, perform functions related to mitotic progression and chromosome segregation. Hence, our analysis predicts structural Features of these proteins and suggests that HEAT repeats may play important roles in chromosome dynamics.