Molecular cloning and expression of cDNA for human RNase H.
Molecular cloning and expression of cDNA for human RNase H.
复制标题
人 RNase H cDNA 的分子克隆和表达。
DOI：
10.1089/oli.1.1998.8.53
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Crooke
中科院分区：
文献类型：
--
作者：
Hongjiang Wu;W. Lima;S. Crooke
We have cloned, expressed, and purified to electrophoretic homogeneity a human RNase H. The enzyme has a molecular weight of 32 kDa, is Mg2+ dependent, and is inhibited by Mn2+ and N-ethylmaleimide. Its molecular weight and cleavage characteristics are consistent with type 2 human RNase H. The human RNase H we have cloned is highly homologous to Escherichia coli RNase HI (33.6% amino acid identity) and to other RNase H enzymes homologous to E. coli RNase HI. The enzyme is encoded by a single gene that is at least 10 kb in length and is expressed ubiquitously in human cells and tissues.
影响因子：
56.9
作者：
YANG, W;HENDRICKSON, WA;SATOW, Y
通讯作者：
SATOW, Y